Detailed information of TA system TA171039

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 110359..110944
• Replicon: plasmid pXM9F202-2-186k
• Accession: NZ_CP060812
• Organism: Acinetobacter variabilis strain XM9F202-2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N9CJF7
• Locus tag: IAQ69_RS15725
• Protein ID: WP_000897307.1
• Coordinates: 110359..110679 (+)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: IAQ69_RS15730
• Protein ID: WP_000369781.1
• Coordinates: 110672..110944 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IAQ69_RS15695	106352..107368	+	1017	WP_005006273.1	hypothetical protein	-
IAQ69_RS15700	107378..107560	-	183	WP_117008140.1	integrase core domain-containing protein	-
IAQ69_RS15705	107573..109219	-	1647	WP_001094417.1	IS66-like element ISAba16 family transposase	-
IAQ69_RS15710	109294..109629	-	336	WP_000618091.1	IS66 family insertion sequence element accessory protein TnpB	-
IAQ69_RS15715	109626..110009	-	384	WP_001055589.1	IS66 family insertion sequence hypothetical protein	-
IAQ69_RS15720	110097..110219	-	123	Protein_106	SMI1/KNR4 family protein	-
IAQ69_RS15725	110359..110679	+	321	WP_000897307.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IAQ69_RS15730	110672..110944	+	273	WP_000369781.1	helix-turn-helix domain-containing protein	Antitoxin
IAQ69_RS15735	111437..112912	+	1476	WP_000052512.1	ABC-F type ribosomal protection protein Msr(E)	-
IAQ69_RS15740	112968..113852	+	885	WP_000155092.1	Mph(E) family macrolide 2'-phosphotransferase	-
IAQ69_RS15745	114090..115229	+	1140	Protein_111	ISNCY family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	tet(M) / sul2 / aph(3')-Ia / aph(3'')-Ib / aph(6)-Id / aac(3)-IId / msr(E) / mph(E) / floR	-	1..186242	186242
	-	inside	IScluster/Tn	aph(3')-Ia / aph(3'')-Ib / aph(6)-Id / aac(3)-IId / msr(E) / mph(E) / floR	-	87389..123016	35627

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (41-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-85)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12187.95 Da
• Isoelectric Point: 7.1639

>T171039 WP_000897307.1 NZ_CP060812:110359-110679 [Acinetobacter variabilis]
MLFIETSIFTKQIKDLVSDEEYRQLQQDLLVQPDRGDLIKNGGGIRKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAY
PKSVKDNLTDKETAILHQLVKEQFHG

Nucleotide

• Length: 321 bp

>T171039 NZ_CP077818:2318280-2318564 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10149.61 Da
• Isoelectric Point: 8.6582

>AT171039 WP_000369781.1 NZ_CP060812:110672-110944 [Acinetobacter variabilis]
MDNNLFDDLVASIKEAGAIKRKEVKASRVTELELPDIKEVREKTGLSQNEFAARLHISPRTLQNWEQGRRYPTGPAATLI
RILDAHPSLI

Nucleotide

• Length: 273 bp

>AT171039 NZ_CP077818:2318048-2318290 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCAGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Toxin

• AlphaFold DB: A0A0A8TMZ3