Detailed information of TA system TA171029

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 4037077..4037674
• Replicon: chromosome
• Accession: NZ_CP060807
• Organism: Klebsiella variicola strain KP2757

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0B7GEF2
• Locus tag: IAP99_RS19595
• Protein ID: WP_012542526.1
• Coordinates: 4037357..4037674 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: IAP99_RS19590
• Protein ID: WP_012542525.1
• Coordinates: 4037077..4037364 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IAP99_RS19560	4032981..4033229	+	249	WP_008805539.1	DUF1158 domain-containing protein	-
IAP99_RS19565	4033246..4033587	-	342	WP_002893035.1	RamA family antibiotic efflux transcriptional regulator	-
IAP99_RS19570	4033618..4034733	-	1116	WP_187726265.1	MBL fold metallo-hydrolase	-
IAP99_RS19575	4034913..4035494	+	582	WP_187726266.1	TetR/AcrR family transcriptional regulator	-
IAP99_RS19580	4035494..4035862	+	369	WP_008805536.1	MmcQ/YjbR family DNA-binding protein	-
IAP99_RS19585	4035982..4036635	+	654	WP_032729541.1	oxygen-insensitive NAD(P)H nitroreductase	-
IAP99_RS19590	4037077..4037364	-	288	WP_012542525.1	helix-turn-helix transcriptional regulator	Antitoxin
IAP99_RS19595	4037357..4037674	-	318	WP_012542526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IAP99_RS19600	4037859..4038902	-	1044	WP_048267621.1	DUF2157 domain-containing protein	-
IAP99_RS19605	4039367..4040233	-	867	WP_008805530.1	helix-turn-helix transcriptional regulator	-
IAP99_RS19610	4040342..4041769	+	1428	WP_187726267.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-89)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12092.35 Da
• Isoelectric Point: 11.2767

>T171029 WP_012542526.1 NZ_CP060807:c4037674-4037357 [Klebsiella variicola]
IFRLVVHVDVKKELQALPPIVQAKMIRQIDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Nucleotide

• Length: 318 bp

>T171029 NZ_CP077816:c3537428-3537087 [Klebsiella pneumoniae]
ATGAAAACTTTACCTGCAACAACTCCGCAGGCGGCGACGCTATGTCTGTCGCCTGTGGCAGTCTGGCAAATGCTGATTGC
ACGCCTGCTGGAACAGCATTATGGCCTGACACTCAACGACACACCATTCAGTGATGAAACGGTAATTCAGGAACATATCG
ATGCCGGTATATCTTTGGCCGATGCCGTTAATTTTCTGGTGGAAAAGTACGGGCTGGTTCGTATTGATCGCAGGGGATTC
AACTGGCAAGAACAGTCCCCATATCTGGGGGCAGTCGATATTCTGAGGGCGCGACAGGCAACTGGTATATTGAAAAGAAA
TCGTATTAGCGCGGCACGGTGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10815.51 Da
• Isoelectric Point: 10.2212

>AT171029 WP_012542525.1 NZ_CP060807:c4037364-4037077 [Klebsiella variicola]
MSKKIIDWDTLKTELLSQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQILLY

Nucleotide

• Length: 288 bp

>AT171029 NZ_CP077816:c3537790-3537449 [Klebsiella pneumoniae]
ATGAAATCTACTGGCTCAGAGAACGATTCATTTCTCCAGTGGGGGCTGAATCGTAATGTGACGCCTTGCTTTGGCGCTCG
TCTGGTACAGGAGGGAAACCGGCTGCATTATCTGGTTGACCGGGCCAGCATTACCGGTAAATTCAGTAACGCAGAATCTT
TGAAATTGGATGAAGTATTCCCTCGTTTTATCAGCCAGATGGAATCGATGTTGACCACTGGTGAAATGAATCCTCGCTAT
GCCCACTGCGTCACCCTGTACCACAACGGTTTCACCTGCGAAGCCGATACACTTGGCAGTTGCGGCTATGTATACATCGC
CGTTTACCCCACTCAACGTTAA

Structures

Toxin

• AlphaFold DB: A0A0B7GEF2