Detailed information of TA system TA170964

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 2022704..2023274
• Replicon: chromosome
• Accession: NZ_CP060789
• Organism: Tessaracoccus defluvii strain JCM 17540

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: H9L22_RS09800
• Protein ID: WP_187719760.1
• Coordinates: 2022993..2023274 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: H9L22_RS09795
• Protein ID: WP_187719759.1
• Coordinates: 2022704..2023006 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H9L22_RS09770	2018584..2019378	+	795	WP_187719755.1	hypothetical protein	-
H9L22_RS09775	2019602..2020633	+	1032	WP_187722651.1	IS30 family transposase	-
H9L22_RS09780	2020630..2021031	+	402	WP_187719756.1	GNAT family N-acetyltransferase	-
H9L22_RS09785	2021479..2022027	-	549	WP_187719757.1	GNAT family N-acetyltransferase	-
H9L22_RS09790	2022183..2022707	-	525	WP_187719758.1	GNAT family N-acetyltransferase	-
H9L22_RS09795	2022704..2023006	-	303	WP_187719759.1	HigA family addiction module antidote protein	Antitoxin
H9L22_RS09800	2022993..2023274	-	282	WP_187719760.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H9L22_RS09805	2023525..2025072	-	1548	WP_187719761.1	Abi family protein	-
H9L22_RS09810	2025687..2026726	-	1040	Protein_1924	IS630 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2010612..2027988	17376
	-	flank	IS/Tn	-	-	2025687..2026214	527

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (24-70)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10926.43 Da
• Isoelectric Point: 9.8455

>T170964 WP_187719760.1 NZ_CP060789:c2023274-2022993 [Tessaracoccus defluvii]
VIRSFGDKATERLWRREPVRSIDPRIQRVALRKLRQVGSAERLDDLRIPPGNRLEALKGDRAGQYSIRINDQWRICFVWT
DAGPEEVEIVDYH

Nucleotide

• Length: 282 bp

>T170964 NZ_CP077806:c3902016-3901633 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTTGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATAACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11002.60 Da
• Isoelectric Point: 5.0747

>AT170964 WP_187719759.1 NZ_CP060789:c2023006-2022704 [Tessaracoccus defluvii]
MTITDKIPPIHPGEVLMEDFIEGFGITQNKLAVSIGVPPRRINEIVHGKRGITADTALRLAKYFGTSAEFWINLQSHYEL
DRAEDLAGEQIASITPLSVA

Nucleotide

• Length: 303 bp

>AT170964 NZ_CP077806:c3902258-3902016 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA