Detailed information of TA system TA170956

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 22503..22929
• Replicon: plasmid pEC96-CTX-M-215
• Accession: NZ_CP060753
• Organism: Escherichia coli strain EC96

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: IAI02_RS23980
• Protein ID: WP_001312861.1
• Coordinates: 22771..22929 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	22503..22727 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IAI02_RS23945	18282..18488	+	207	WP_073520019.1	single-stranded DNA-binding protein	-
IAI02_RS23950	18514..18966	+	453	WP_181695943.1	single-stranded DNA-binding protein	-
IAI02_RS23955	19029..19262	+	234	WP_087086828.1	DUF905 family protein	-
IAI02_RS23960	19328..21286	+	1959	WP_099357091.1	ParB/RepB/Spo0J family partition protein	-
IAI02_RS23965	21341..21775	+	435	WP_000845949.1	conjugation system SOS inhibitor PsiB	-
IAI02_RS23970	21772..22491	+	720	WP_001276234.1	plasmid SOS inhibition protein A	-
IAI02_RS23975	22503..22691	-	189	WP_001299721.1	hypothetical protein	-
-	22503..22727	+	225	NuclAT_0	-	Antitoxin
-	22503..22727	+	225	NuclAT_0	-	Antitoxin
-	22503..22727	+	225	NuclAT_0	-	Antitoxin
-	22503..22727	+	225	NuclAT_0	-	Antitoxin
IAI02_RS23980	22771..22929	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
IAI02_RS23985	23850..24137	+	288	WP_000107535.1	hypothetical protein	-
IAI02_RS23990	24255..25076	+	822	WP_073520018.1	DUF945 domain-containing protein	-
IAI02_RS23995	25373..26020	-	648	WP_000614935.1	transglycosylase SLT domain-containing protein	-
IAI02_RS24000	26297..26680	+	384	WP_000124981.1	relaxosome protein TraM	-
IAI02_RS24005	26871..27557	+	687	WP_073520017.1	PAS domain-containing protein	-
IAI02_RS24010	27651..27878	+	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-125	-	1..68439	68439

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T170956 WP_001312861.1 NZ_CP060753:22771-22929 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T170956 NZ_CP077805:4816141-4816359 [Klebsiella variicola]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAACTGGCGGTATTTTACTCAGCCGCCGATCATCGTCTGGCTGAAC
TGACCATGAATAAGCTGTACGATAAGATCCCGACCTCAGTCTGGAAGTTTATCCGCTAA

Antitoxin

Nucleotide

• Length: 225 bp

>AT170956 NZ_CP060753:22503-22727 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin