Detailed information of TA system TA170955

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 21058..21710
• Replicon: plasmid pEC96-32kb
• Accession: NZ_CP060752
• Organism: Escherichia coli strain EC96

Toxin (Protein)

• Gene name: higB
• Uniprot ID: B2CBE9
• Locus tag: IAI02_RS23755
• Protein ID: WP_012457127.1
• Coordinates: 21360..21710 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: B2CBE8
• Locus tag: IAI02_RS23750
• Protein ID: WP_012457126.1
• Coordinates: 21058..21357 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IAI02_RS23725	17094..17849	+	756	WP_023316386.1	hypothetical protein	-
IAI02_RS23730	17952..18482	+	531	WP_012457122.1	phospholipase D family protein	-
IAI02_RS23735	18666..19634	-	969	WP_012457123.1	DUF1738 domain-containing protein	-
IAI02_RS23740	20019..20684	+	666	WP_094309421.1	hypothetical protein	-
IAI02_RS23745	20732..21004	-	273	WP_187476165.1	hypothetical protein	-
IAI02_RS23750	21058..21357	-	300	WP_012457126.1	helix-turn-helix domain-containing protein	Antitoxin
IAI02_RS23755	21360..21710	-	351	WP_012457127.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IAI02_RS23760	22104..22721	+	618	WP_012457128.1	ParA family protein	-
IAI02_RS23765	22776..23015	+	240	WP_012457129.1	ParG	-
IAI02_RS23770	23634..24890	-	1257	WP_117061474.1	GIY-YIG nuclease family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..32368	32368

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-107)

Antitoxin

• Cro/C1-type helix-turn-helix domain (34-83)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13378.26 Da
• Isoelectric Point: 6.7130

>T170955 WP_012457127.1 NZ_CP060752:c21710-21360 [Escherichia coli]
MWDIITTECFDGWFLAQSDALRESVYEAMGVLEKFGPALGRPYVDTLNDSDFANMKELRIQHHGNPVRAFFAFDPTRRAI
VLCAGDKTGANEKRFYKDMIRLADSEYRKHLAKLEK

Nucleotide

• Length: 351 bp

>T170955 NZ_CP077805:c4687343-4687026 [Klebsiella variicola]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAAATAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTATGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11011.75 Da
• Isoelectric Point: 5.8719

>AT170955 WP_012457126.1 NZ_CP060752:c21357-21058 [Escherichia coli]
MATYRELLAKESPEMQARVAERVEEVSIQIALSMLRDELNISQTELAAVMGVKQPSVARMEQADNDPRLSTLKRYVKALG
GELSLDVTLPTGKRVAFHI

Nucleotide

• Length: 300 bp

>AT170955 NZ_CP077805:c4687033-4686746 [Klebsiella variicola]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTAGCCAGCCTGAGGTTCAGGCTGCGTTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTGACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACGGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGTGGAATAAAGCACCCACAAATTTTACTTTACTAA

Structures

Toxin

• AlphaFold DB: B2CBE9

Antitoxin

• AlphaFold DB: B2CBE8