Detailed information of TA system TA170950

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3616520..3617214
• Replicon: chromosome
• Accession: NZ_CP060748
• Organism: Escherichia coli strain EC96

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: IAI02_RS17560
• Protein ID: WP_001263493.1
• Coordinates: 3616520..3616918 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: IAI02_RS17565
• Protein ID: WP_000554757.1
• Coordinates: 3616921..3617214 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IAI02_RS17530	3611520..3612764	-	1245	WP_000189541.1	esterase FrsA	-
-	3612180..3612260	-	81	NuclAT_9	-	-
-	3612180..3612260	-	81	NuclAT_9	-	-
-	3612180..3612260	-	81	NuclAT_9	-	-
-	3612180..3612260	-	81	NuclAT_9	-	-
IAI02_RS17535	3612856..3613314	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
IAI02_RS17540	3613575..3615032	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
IAI02_RS17545	3615089..3615610	-	522	Protein_3432	peptide chain release factor H	-
IAI02_RS17550	3615609..3615812	-	204	Protein_3433	RNA ligase RtcB family protein	-
IAI02_RS17555	3616058..3616510	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
IAI02_RS17560	3616520..3616918	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
IAI02_RS17565	3616921..3617214	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
IAI02_RS17570	3617266..3618321	-	1056	WP_001226164.1	DNA polymerase IV	-
IAI02_RS17575	3618392..3619177	-	786	WP_000207564.1	putative lateral flagellar export/assembly protein LafU	-
IAI02_RS17580	3619122..3620861	+	1740	WP_001334802.1	flagellar biosynthesis protein FlhA	-
IAI02_RS17585	3621085..3621582	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3597405..3625469	28064

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T170950 WP_001263493.1 NZ_CP060748:c3616918-3616520 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T170950 NZ_CP077805:1384806-1385291 [Klebsiella variicola]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATTACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGACCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTCAT
GCCTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT170950 WP_000554757.1 NZ_CP060748:c3617214-3616921 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT170950 NZ_CP077805:1384516-1384809 [Klebsiella variicola]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACCGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGTTAA
TTGAGCAACGTATCATCATGGCGGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6