Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 739245..739972 Replicon chromosome
Accession NZ_CP060748
Organism Escherichia coli strain EC96

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag IAI02_RS03610 Protein ID WP_000550189.1
Coordinates 739245..739559 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag IAI02_RS03615 Protein ID WP_000560266.1
Coordinates 739556..739972 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IAI02_RS03590 735403..736389 - 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -
IAI02_RS03595 736468..737160 - 693 WP_000942548.1 vancomycin high temperature exclusion protein -
IAI02_RS03600 737237..737740 - 504 WP_001333820.1 M48 family metallopeptidase -
IAI02_RS03605 737825..738961 + 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
IAI02_RS03610 739245..739559 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
IAI02_RS03615 739556..739972 + 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
IAI02_RS03620 740017..742035 - 2019 WP_000121433.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
IAI02_RS03625 742461..744812 - 2352 WP_000695487.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T170934 WP_000550189.1 NZ_CP060748:739245-739559 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T170934 NZ_CP077803:105336-105770 [Rhodospirillum rubrum]
TTGTATCTGGTCGACACCAACGTTCTGTCGGCCAGGGCGCCGTCACGGGTCGCCCCGGTCGAACTGATCGCCTGGATGGA
CGCCCATTCGGCGGAGCTTTTCCTCTCGGCGGTGACGGTGGCGGAAATCGAGGACGGCATCGCCAAGCTGCGTCGCGAAG
GCGCGACGCGCAAGGCGGCCGATCTGATGGCATGGCTGGACGCGCTGCTGCATCTCTATGGCAACCGCATCCTGCCGTTC
GACACGACCCTCGCGCGCCTGGCCGGCGTGCTTTCCGACCGGGCGCGGGCGCAAGGCCAGGCGCCGGGTTTTGCCGACAT
CGCCATCGCGGCGACGGCCAAACACCACGACCTGACGATCCTGACCCGCAACGTTCGCCACTTCGACCGCTTGGGCGTGC
CGGTGATCGATCCCTTCGCGGCGTTGCCCCGGTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT170934 WP_000560266.1 NZ_CP060748:739556-739972 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT170934 NZ_CP077803:105090-105332 [Rhodospirillum rubrum]
ATGCGCGAAATTCAGCTTCGGGACGCCAAGGCCCGCCTGTCGGCGGTGGTTGACGACGCGGTGCGCGGCGAGCCGGCTAT
CATCACCCGGCATGGCAAGCGGGAGGCCGTGGTTCTGAGCTTCGCCGAGTGGGAGCGTCTTTCACAGGTGCCCTCTTTCG
GCCGTTTGCTGATGAGCGCGCCGCTGGCGCCCGACGATCTGCCGCCCCGAAACGAAGCGCCGCTGCGCGATGCCGGGCTC
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References