Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 695900..696699 Replicon chromosome
Accession NZ_CP060748
Organism Escherichia coli strain EC96

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag IAI02_RS03390 Protein ID WP_000347273.1
Coordinates 695900..696364 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag IAI02_RS03395 Protein ID WP_001307405.1
Coordinates 696364..696699 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IAI02_RS03360 690901..691335 - 435 WP_000948842.1 PTS sugar transporter subunit IIA -
IAI02_RS03365 691353..692231 - 879 WP_001295548.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
IAI02_RS03370 692221..693000 - 780 WP_000406209.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
IAI02_RS03375 693011..693484 - 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
IAI02_RS03380 693507..694787 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
IAI02_RS03385 695036..695845 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
IAI02_RS03390 695900..696364 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
IAI02_RS03395 696364..696699 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
IAI02_RS03400 696848..698419 - 1572 WP_001273753.1 galactarate dehydratase -
IAI02_RS03405 698794..700128 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
IAI02_RS03410 700144..700914 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T170933 WP_000347273.1 NZ_CP060748:c696364-695900 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T170933 NZ_CP077802:55379-55699 [Acinetobacter baumannii]
ATGTTATTCATTGAAACCAGTATTTTTACCAAGCAAATCAAAGAGCTTGTTACTGATGAAGAATATCGTCAACTCCAGCA
AGATCTATTGATACAACCTGATAAAGGAGACTTGATCAAAAATGGTGGTGGCATTCGTAAAGTCCGTTGTGCTCAGGGTA
ATAAAGGCAAAAGTGGCGGTATTCGTGTGATCTATTACTGGGTTACAGAGGATGATCAGATTTTTTTCCTAGTGGCTTAT
CCAAAGTCTGTGAAAGATAATCTGACAGACAAAGAGACCTCGATCCTGCGTCAATTAGTGAAGGAGCAATTTAATGGATA
A

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT170933 WP_001307405.1 NZ_CP060748:c696699-696364 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT170933 NZ_CP077802:55692-55964 [Acinetobacter baumannii]
ATGGATAACAACTTATTTGATGACCTCGTTACTTCCATCAAAGAAGCTGGAGCCATCAAACGTAATGAGATAAAGGCAAG
TCGAGTAACAGAACTTGAATTGCCTGATATTAAAGAAGTACGTGAGAAAACAGGCTTAACCCAGGCAGAGTTTGCTGCAC
GCTTACATATTAGCGCGAGAACTCTACAAAATTGGGAACAAGGTCGTCGCTATCCGACTGGCCCAGCAGCGACTTTGATT
CGTATTCTTGATACCAATCCAACTCTGATTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References