Detailed information of TA system TA170930

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 23243..23512
• Replicon: plasmid pKP120-CTX-M-125
• Accession: NZ_CP060746
• Organism: Klebsiella pneumoniae strain KP120

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: IAI03_RS26975
• Protein ID: WP_001312861.1
• Coordinates: 23354..23512 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	23243..23308 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IAI03_RS26945	18865..19071	+	207	WP_073520019.1	single-stranded DNA-binding protein	-
IAI03_RS26950	19097..19549	+	453	WP_181695943.1	single-stranded DNA-binding protein	-
IAI03_RS26955	19612..19845	+	234	WP_087086828.1	DUF905 family protein	-
IAI03_RS26960	19911..21869	+	1959	WP_099357091.1	ParB/RepB/Spo0J family partition protein	-
IAI03_RS26965	21924..22358	+	435	WP_000845949.1	conjugation system SOS inhibitor PsiB	-
IAI03_RS26970	22355..23074	+	720	WP_001276234.1	plasmid SOS inhibition protein A	-
-	23086..23310	+	225	NuclAT_0	-	-
-	23086..23310	+	225	NuclAT_0	-	-
-	23086..23310	+	225	NuclAT_0	-	-
-	23086..23310	+	225	NuclAT_0	-	-
-	23243..23308	-	66	-	-	Antitoxin
IAI03_RS26975	23354..23512	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
IAI03_RS26980	24433..24720	+	288	WP_000107535.1	hypothetical protein	-
IAI03_RS26985	24838..25659	+	822	WP_073520018.1	DUF945 domain-containing protein	-
IAI03_RS26990	25956..26603	-	648	WP_000614935.1	transglycosylase SLT domain-containing protein	-
IAI03_RS26995	26880..27263	+	384	WP_000124981.1	relaxosome protein TraM	-
IAI03_RS27000	27454..28140	+	687	WP_073520017.1	PAS domain-containing protein	-
IAI03_RS27005	28234..28461	+	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-125	-	1..72636	72636

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T170930 WP_001312861.1 NZ_CP060746:23354-23512 [Klebsiella pneumoniae]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T170930 NZ_CP077784:270325-270609 [Klebsiella pneumoniae]
ATGACCTATAAGCTGGCCTTTAATGAATCAGCTCTGAAAGAATGGAAAAAATTGGGCCATACCATTCGGGAACAATTCAA
AAAGAAGCTCGCTGAGCGCCTGTTGAACCCACGGGTGCCCGCATCGCAACTCCATGGACGCAAAGACCAGTACAAAATAA
AGCTCCGTGGCGCAGGCTACCGACTGGTTTACAGTGTCAACGATGATGTTGTAACGGTCACCGTAATTGGCGTCGGTAAA
CGTGAGAATGATGATATTTACAATTTGACCAAACACAGAAATTAA

Antitoxin

Nucleotide

• Length: 66 bp

>AT170930 NZ_CP060746:c23308-23243 [Klebsiella pneumoniae]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin