Detailed information of TA system TA170915

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 1415413..1416010
• Replicon: chromosome
• Accession: NZ_CP060744
• Organism: Klebsiella pneumoniae strain KP120

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A9J6S5A1
• Locus tag: IAI03_RS06725
• Protein ID: WP_004893639.1
• Coordinates: 1415413..1415730 (+)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: R4YH91
• Locus tag: IAI03_RS06730
• Protein ID: WP_004142561.1
• Coordinates: 1415723..1416010 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IAI03_RS06710	1411113..1412540	-	1428	WP_004176980.1	MFS transporter	-
IAI03_RS06715	1412649..1413515	+	867	WP_180995921.1	helix-turn-helix transcriptional regulator	-
IAI03_RS06720	1414185..1415228	+	1044	WP_023279204.1	DUF2157 domain-containing protein	-
IAI03_RS06725	1415413..1415730	+	318	WP_004893639.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IAI03_RS06730	1415723..1416010	+	288	WP_004142561.1	helix-turn-helix transcriptional regulator	Antitoxin
IAI03_RS06735	1416148..1416801	-	654	Protein_1300	oxygen-insensitive NAD(P)H nitroreductase	-
IAI03_RS06740	1416921..1417289	-	369	WP_004142557.1	MmcQ/YjbR family DNA-binding protein	-
IAI03_RS06745	1417289..1417870	-	582	WP_004176968.1	TetR/AcrR family transcriptional regulator	-
IAI03_RS06750	1418050..1419165	+	1116	WP_074180844.1	MBL fold metallo-hydrolase	-
IAI03_RS06755	1419196..1419537	+	342	WP_002893035.1	RamA family antibiotic efflux transcriptional regulator	-
IAI03_RS06760	1419555..1419803	-	249	WP_002893037.1	DUF1158 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-88)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12100.35 Da
• Isoelectric Point: 11.2767

>T170915 WP_004893639.1 NZ_CP060744:1415413-1415730 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPSIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Nucleotide

• Length: 318 bp

>T170915 NZ_CP077778:c143124-142681 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10771.46 Da
• Isoelectric Point: 10.2212

>AT170915 WP_004142561.1 NZ_CP060744:1415723-1416010 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Nucleotide

• Length: 288 bp

>AT170915 NZ_CP077778:c143351-143121 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Antitoxin

• AlphaFold DB: A0A0S3DIQ1