Detailed information of TA system TA170845

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symE-ohsC/SymE(toxin)
• Location: 4763533..4763945
• Replicon: chromosome
• Accession: NZ_CP060709
• Organism: Escherichia coli strain ST18

Toxin (Protein)

• Gene name: symE
• Uniprot ID: U9YSY7
• Locus tag: H9445_RS23240
• Protein ID: WP_000132601.1
• Coordinates: 4763533..4763874 (-)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	ohsC
Locus tag	-
Coordinates	4763869..4763945 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H9445_RS23230	4760946..4761992	-	1047	WP_000437621.1	5-methylcytosine-specific restriction endonuclease system specificity protein McrC	-
H9445_RS23235	4761992..4763371	-	1380	WP_000443951.1	5-methylcytosine-specific restriction endonuclease subunit McrB	-
H9445_RS23240	4763533..4763874	-	342	WP_000132601.1	endoribonuclease SymE	Toxin
-	4763869..4763945	+	77	NuclAT_14	-	Antitoxin
-	4763869..4763945	+	77	NuclAT_14	-	Antitoxin
-	4763869..4763945	+	77	NuclAT_14	-	Antitoxin
-	4763869..4763945	+	77	NuclAT_14	-	Antitoxin
-	4763869..4763945	+	77	NuclAT_15	-	Antitoxin
-	4763869..4763945	+	77	NuclAT_15	-	Antitoxin
-	4763869..4763945	+	77	NuclAT_15	-	Antitoxin
-	4763869..4763945	+	77	NuclAT_15	-	Antitoxin
H9445_RS23245	4764102..4765496	-	1395	WP_001272447.1	type I restriction-modification system specificity subunit	-
H9445_RS23250	4765493..4767082	-	1590	WP_001063204.1	type I restriction-modification system methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4739270..4765496	26226

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12203.02 Da
• Isoelectric Point: 8.5012

>T170845 WP_000132601.1 NZ_CP060709:c4763874-4763533 [Escherichia coli]
MTDTHSIAQPFEAEVSPANNRHVTVGYASRYPDYSRIPAITLKGQWLEAAGFATGTAVDVKVMEGCIVLTAQPPAAEESE
LMQSLRQVCKLSARKQKQVQAFIGVIAGKQKVA

Nucleotide

• Length: 342 bp

>T170845 NZ_CP077738:c925682-925494 [Staphylococcus aureus]
ATGTCTTTACATTTTGCAATTCTGTTTTGGCTAGCATTAATTTTCTTAGTTGCCGCTACGTTTATACTCGTATTAATGAA
AAAAACTGGCAAAGAATCTAAAAAAGAGTCCTATTTAAGTTTCACTGTCATTCTCTATATTTTTGGATTCGCTATATTAA
TATACACATTTATATTTGGTGTGCTATAA

Antitoxin

Nucleotide

• Length: 77 bp

>AT170845 NZ_CP060709:4763869-4763945 [Escherichia coli]
AGTCATAACTGCTATTCTCCAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: U9YSY7

Antitoxin