Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3437279..3438078 Replicon chromosome
Accession NZ_CP060709
Organism Escherichia coli strain ST18

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag H9445_RS16950 Protein ID WP_000347273.1
Coordinates 3437614..3438078 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag H9445_RS16945 Protein ID WP_001307405.1
Coordinates 3437279..3437614 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9445_RS16930 3433064..3433834 - 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -
H9445_RS16935 3433850..3435184 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
H9445_RS16940 3435559..3437130 + 1572 WP_001273747.1 galactarate dehydratase -
H9445_RS16945 3437279..3437614 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
H9445_RS16950 3437614..3438078 + 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
H9445_RS16955 3438133..3438942 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
H9445_RS16960 3439191..3440471 + 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
H9445_RS16965 3440494..3440967 + 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
H9445_RS16970 3440978..3441349 + 372 Protein_3321 PTS sugar transporter subunit IIC -
H9445_RS16975 3441345..3441902 + 558 Protein_3322 amidohydrolase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3428131..3450400 22269


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T170839 WP_000347273.1 NZ_CP060709:3437614-3438078 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T170839 NZ_CP077717:1943711-1944325 [Saccharolobus shibatae B12]
ATGTTAAAATCACCGTTTGAAGTAACAATCTTGATTGAAGAGCACCCTTGTGAGGTTATGAGGATAATATCTAGTACTGG
GCTTAAAGCAACTGTTGACAACGTTAAACTCGGGGATAATGCAACTGATCACATAGTCCTTTTCGAAAAAGAGGTCCAAA
AAGATGATCTAATTAAACTTAAGTCCCATAGTACCAAAGTCTTAAAGCTTAATGATAGCAAAGTATGGGTTAGAACTAAT
GGATGCTCAGTTTGTAAGATTCTCTATACTTCAAATGTTGTCGTAGAGAAAATAAAAGTAGTAAAAGAGAGAACATTACT
CTATACGCTTTTGGTCCCTAATACGATAGCTTTTAAGGAGTTTCTAGCTAATCTTACTAGTCAAGGGATAAAGTTTACAG
TCCTAAACACCTCTGAAATAAGCAGTAATGACTTAACTGATAGACAAATGGAAATATTGAAACTTGCATACAAGATGGGT
TATTTTGATGATGATAGGAGAGTAACTTTAACTGAATTAGCAAAGCAATTGGGAATATCAGCTCCGACTTTGGAGGAAAT
TATGAGGAGAGCGTTAAGGAAAGTCGTCAAGTATTATTTAGATAAAGTAGGGTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT170839 WP_001307405.1 NZ_CP060709:3437279-3437614 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT170839 NZ_CP077717:1943346-1943693 [Saccharolobus shibatae B12]
ATGATTAAGATAAAGAGGGTTTACGATCCAATAGAGAAAGATGACGGAATAAGAATCTTAGTGGATAGGTTATGGCCAAG
AGGAATTGGCAAAAATAAGGTAGATTTATGGCTTAAAGATATAGCACCTAGTGATGAGTTACGAAGATGGTACGGTCATG
ATGCCAGTAAATGGGATGAGTTCAAAAGGAAATATTTCGAGGAACTAGATGCTAATCCAAAGATGAGTATTTTATTGCAA
TTAATTAGAAAGGGCGAAAATATTACCTTACTTTACGCCTCAAAATCACCGTATAATAATGCAATTGCGTTAAAGGAGTA
TCTAGAAAAAAAGATATTAAAACAATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References