Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2251455..2251957 Replicon chromosome
Accession NZ_CP060709
Organism Escherichia coli strain ST18

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag H9445_RS11400 Protein ID WP_000767829.1
Coordinates 2251455..2251709 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag H9445_RS11405 Protein ID WP_001259255.1
Coordinates 2251706..2251957 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9445_RS11370 2246470..2246697 - 228 WP_000234896.1 sulfurtransferase TusA -
H9445_RS11375 2246711..2247769 - 1059 WP_000492339.1 transport protein YeeE -
H9445_RS11380 2247948..2249306 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
H9445_RS11385 2249296..2249358 - 63 WP_010723108.1 membrane protein YoeI -
H9445_RS11390 2249573..2250502 - 930 WP_000803351.1 LysR family transcriptional regulator -
H9445_RS11395 2250548..2251372 - 825 WP_000754737.1 SDR family oxidoreductase -
H9445_RS11400 2251455..2251709 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
H9445_RS11405 2251706..2251957 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
H9445_RS11410 2252240..2252290 + 51 WP_001364200.1 his operon leader peptide -
H9445_RS11415 2252436..2253335 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
H9445_RS11420 2253341..2254645 + 1305 WP_000009594.1 histidinol dehydrogenase -
H9445_RS11425 2254642..2255712 + 1071 WP_000108941.1 histidinol-phosphate transaminase -
H9445_RS11430 2255712..2256779 + 1068 WP_000080105.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T170832 WP_000767829.1 NZ_CP060709:c2251709-2251455 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T170832 NZ_CP077715:1290320-1290712 [Saccharolobus shibatae]
ATGATAGTAGTTGATACTAACGTTTTAGTTTACGCTACATTCGAGGACTCTAAATATCATAAGGAAAGTGTTAATATTAT
ACAAAGGGAAGACGTGATAATTCCACAAATAGTCGTTTATGAGTATCTAAAAGTGTTAAGTGAGCTGGTTCCGGATTTGA
ACTTTGTAAAAACAAAGACTGAGGAATTAACAGAGTTTAATATAGTGTGTGAGGATATGAATATAATATTACAAGCTATT
AATCTTATTAAAAATACAAAACTCTCTCTAAAAAACATTAATGATTTTATAATTCTCTCTTTCTCTCTCTATACAAAGAG
TTCCTTAGCAACATTCGATGTAAAACTGAGGAAATTAGCTGAAAAGAATGGTGTCAATGTTCTTCCAAGCTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT170832 WP_001259255.1 NZ_CP060709:c2251957-2251706 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT170832 NZ_CP077715:1290100-1290333 [Saccharolobus shibatae]
ATGAAAAGGAACGTGTCGAAAGTTACTAGGAATTATCAATTAACTATACCTCTTGAGATTAGGGAGTTTCTCGGTATAAA
AGAAGGTGAATATGTTGAGTTTGAGATTGATGATGGGAAGGTTGTAATTAAACCCCTTAGAAAGAAGTGGAGTACTGTGA
AACTGCGTAAACAAGTAAGTGTTGAAGAAATTGAAGAAATCGCTAACAGTGCATTTGAAAATGATAGTAGTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References