Detailed information of TA system TA170816

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 1575377..1575748
• Replicon: chromosome
• Accession: NZ_CP060709
• Organism: Escherichia coli strain ST18

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: F4VC37
• Locus tag: H9445_RS07990
• Protein ID: WP_001317028.1
• Coordinates: 1575554..1575748 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	1575377..1575555 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H9445_RS07965	1571332..1572705	+	1374	WP_000123737.1	ATP-dependent RNA helicase DbpA	-
H9445_RS07970	1572834..1573769	-	936	WP_001157406.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
H9445_RS07975	1573821..1575056	-	1236	WP_000040852.1	site-specific integrase	-
H9445_RS07980	1575058..1575273	-	216	WP_000079604.1	excisionase XisR	-
H9445_RS07985	1575352..1575561	-	210	WP_000276809.1	double-strand break reduction protein RcbA	-
-	1575377..1575555	+	179	NuclAT_0	-	Antitoxin
-	1575377..1575555	+	179	NuclAT_0	-	Antitoxin
-	1575377..1575555	+	179	NuclAT_0	-	Antitoxin
-	1575377..1575555	+	179	NuclAT_0	-	Antitoxin
H9445_RS07990	1575554..1575748	-	195	WP_001317028.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
H9445_RS07995	1575805..1576614	-	810	WP_000166319.1	recombination protein RecT	-
H9445_RS08000	1576607..1579207	-	2601	WP_000105143.1	exodeoxyribonuclease VIII	-
H9445_RS08005	1579309..1579584	-	276	WP_000632297.1	protein RacC	-
H9445_RS08010	1579659..1579829	-	171	WP_001352098.1	conserved protein, Rac prophage	-
H9445_RS08015	1579829..1580050	-	222	WP_000560225.1	killing protein KilR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1544993..1595495	50502

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7018.89 Da
• Isoelectric Point: 8.9538

>T170816 WP_001317028.1 NZ_CP060709:c1575748-1575554 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T170816 NZ_CP077710:5034898-5035209 [Salmonella enterica subsp. enterica]
GTGCATGTTATCAGCCGAAAACCGTTCAATGAAGCGATGCTCATGTATCCAAACCATGAGCTTGCCCTCACTGAGTTGCT
GAATGTTCTTGAGAAAAAAACATTCACTCAGCCTGAAGAGATGAAGCGGTATATTCCTTCACTGGATAATTTTAAGTACA
GAGATAAATGGTGGGTGATTGATGTTTCTGGTAATAGCCTGAGACTCATTTCCTACATAGATTTCAGGTTACATAAGATA
TTTGTGAAACATATCGTTTCCCATGCCGAATATGACAAACTGACCGCATATTATCGGGGTAATAAAGAATGA

Antitoxin

Nucleotide

• Length: 179 bp

>AT170816 NZ_CP060709:1575377-1575555 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A7L6LHV7

Antitoxin