Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 245960..246501 Replicon chromosome
Accession NZ_CP060709
Organism Escherichia coli strain ST18

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag H9445_RS01140 Protein ID WP_000615983.1
Coordinates 245960..246238 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag H9445_RS01145 Protein ID WP_000729703.1
Coordinates 246241..246501 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9445_RS01125 243542..244120 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
H9445_RS01130 244326..245093 + 768 WP_000333380.1 class II glutamine amidotransferase -
H9445_RS01135 245064..245804 - 741 WP_001225679.1 murein L,D-transpeptidase -
H9445_RS01140 245960..246238 - 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
H9445_RS01145 246241..246501 - 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
H9445_RS01150 246711..247460 + 750 WP_000056849.1 C40 family peptidase -
H9445_RS01155 247636..248133 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
H9445_RS01160 248357..250096 - 1740 WP_001334802.1 flagellar biosynthesis protein FlhA -
H9445_RS01165 250041..250826 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - gmhA/lpcA 221614..281920 60306


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T170807 WP_000615983.1 NZ_CP060709:c246238-245960 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T170807 NZ_CP077710:641422-641697 [Salmonella enterica subsp. enterica]
ATGAAACAGGTATTAAAAGATTTTGACGTCGCAGACTATTTAGAGGACGAGGATGATGTTATCGCTTATCTAAATGCCGT
TATTGCCGAGGGTGACAATAAACAACTTTTCATTGCGCTGGGCAACATTGCAAGAGCAAGAAATATAAGCCGTATTTCAA
AAGAAACCGGAATCAGCAGGGAGGGAATTTATAAAGCACTTTCAGGCGATGGAAATCCATCATTTAACACCCTTTCTAAA
ATAACTAAAGCGATGGGGTTAGATCTTCACTTTTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT170807 WP_000729703.1 NZ_CP060709:c246501-246241 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT170807 NZ_CP077710:641734-642072 [Salmonella enterica subsp. enterica]
ATGACCTATCGCGAAGATATTCGGTTGGTGGAGTATCTGATAGAGCACGAGCCGGATAGCCCGCTGATTGACATGCTCAC
AGCAAAAATTGACCGTTACGAAGATGAAGCGCCGGAGCTGGCAGAGTTTAACGCCAATATAGCCAGCACGCCGGGCGGCG
TGGCAATGTTGCGCGTACTTATGGATCAATACCATCTTGCACAAGGTAACTTTGAAAACGAGATAGGCAAGCGCTCGCTG
GTATGTCGGATACTTAACGGGCAGCGCAGGCTAACCCTTGATCATGCGCGTGCGCTGGCCAGAAGGTTTAATCTGCCTGT
AAGCGCGTTTATTGATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References