Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3218460..3219187 Replicon chromosome
Accession NZ_CP060708
Organism Escherichia coli strain MFDpir

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag H9446_RS15620 Protein ID WP_000550189.1
Coordinates 3218873..3219187 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag H9446_RS15615 Protein ID WP_000560266.1
Coordinates 3218460..3218876 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9446_RS15605 3213620..3215971 + 2352 WP_000695487.1 alpha-glucosidase -
H9446_RS15610 3216397..3218415 + 2019 WP_000121433.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
H9446_RS15615 3218460..3218876 - 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
H9446_RS15620 3218873..3219187 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
H9446_RS15625 3219471..3220607 - 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
H9446_RS15630 3220692..3221195 + 504 WP_001333820.1 M48 family metallopeptidase -
H9446_RS15635 3221272..3221964 + 693 WP_000942548.1 vancomycin high temperature exclusion protein -
H9446_RS15640 3222043..3223029 + 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T170796 WP_000550189.1 NZ_CP060708:c3219187-3218873 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T170796 NZ_CP077706:c2089547-2089365 [Salmonella enterica subsp. enterica]
TTGAAAAGCTCGGAGTTGATAAAACTACTGGAAAGCAAAGGGTGGACGCTGGAACGAATTAACGGTAGTCATCACCATTT
TTCGCACCCTGACTTTCCTGTGATTATTACGGTTCCTCATCCCAGGAAAGATATAAAACCCGGAACGCTTAACCAGATAC
TGAAAAAGGCAAAGCTAAAGTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT170796 WP_000560266.1 NZ_CP060708:c3218876-3218460 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT170796 NZ_CP077706:c2089322-2088909 [Salmonella enterica subsp. enterica]
ATGTTGTATCCTGCTTTTATCGAAGTGGATAAAGACGGTAGCGCCAGCGGGTGGTTTCCCGATGTTCCCGGCTGTATTTT
CGCCGGCAGCACTATTGAGGAAACCTATGCCGACGCACGCAGCGCTATCTGTGCTCATTTTGAAGTGCTCTGTGATAAGG
GGTTTGATATTCCCTCTCCCCGCAGCATCGAGCAACATGTCGCCGCCGAACCAGACGATTACAGTAACGGACGCTGGTTG
TATGTGGACGTAAATATGGATCAGTTTGACGGGAGAGCTGAACGCATCAACGTCACTCTTCCCCATCGCCTGCTCGATCG
CATCGACGCCACGGTAAAACAGCATCCCGACTACGGTAGCCGGAGTGCATTTCTGGCAGCAGCTGCGCGTAATGAGTTAC
AGAAATCGCTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References