Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2072885..2073387 Replicon chromosome
Accession NZ_CP060708
Organism Escherichia coli strain MFDpir

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag H9446_RS10265 Protein ID WP_000767829.1
Coordinates 2072885..2073139 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag H9446_RS10270 Protein ID WP_001259255.1
Coordinates 2073136..2073387 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9446_RS10235 2067900..2068127 - 228 WP_000234896.1 sulfurtransferase TusA -
H9446_RS10240 2068141..2069199 - 1059 WP_000492339.1 transport protein YeeE -
H9446_RS10245 2069378..2070736 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
H9446_RS10250 2070726..2070788 - 63 WP_010723108.1 membrane protein YoeI -
H9446_RS10255 2071003..2071932 - 930 WP_000803351.1 LysR family transcriptional regulator -
H9446_RS10260 2071978..2072802 - 825 WP_000754737.1 SDR family oxidoreductase -
H9446_RS10265 2072885..2073139 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
H9446_RS10270 2073136..2073387 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
H9446_RS10275 2073670..2073720 + 51 WP_001364200.1 his operon leader peptide -
H9446_RS10280 2073866..2074765 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
H9446_RS10285 2074771..2076075 + 1305 WP_000009594.1 histidinol dehydrogenase -
H9446_RS10290 2076072..2077142 + 1071 WP_000108941.1 histidinol-phosphate transaminase -
H9446_RS10295 2077142..2078209 + 1068 WP_000080105.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T170790 WP_000767829.1 NZ_CP060708:c2073139-2072885 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T170790 NZ_CP077704:c4653549-4653238 [Salmonella enterica subsp. enterica]
ATGCAATTTATAGAAACGGAACTCTTTACTGAAGATGTTAAAAAACTGCTCGATGAGGATGAATACCATAAGCTTCAGGT
TTTTATGGCTCAGCATCCAGATTGTGGTGATGTCATTCAGGAAACGGGCGGCCTGAGAAAAATGCGCTGGGGAGCGCGAG
GCAAAGGAAAGCGTAGTGGCGTGCGAATTATCTATTTTCACCGTAGTCAACGGTATGAGATTCGCTTGCTTCTGATTTAT
CAAAAAGGCATTAAAGATGATCTCACGCCGCAGGAAAAAGCGGTGCTTCGTATGCTGAATGAGAGGTGGTAG

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT170790 WP_001259255.1 NZ_CP060708:c2073387-2073136 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT170790 NZ_CP077704:c4653237-4652947 [Salmonella enterica subsp. enterica]
ATGGATAAAGTGTTATTTGAGCGATTAACTCAAAGTATGTCTCAAATGAATGAAATCATTGAAGGGACCCGTGAGCCTTC
TCGTACCTTTCATATTGATGCAATGAAGATTAAAGAGATACGGCAGGCATCTGGGTTGTCGCAATCTAAGTTTGCAGAGC
TGATTTCGGTCAACGTGGATACGCTGCGCAACTGGGAGCAAGGAAGACGTTCACCGACAGGGCCAGCCAAAGCGTTACTT
CGCGCCATTGCCAACGACCCGAGAAACGTTATACAAGCATTGCGTTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References