Detailed information of TA system TA170765

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 252005..252699
• Replicon: chromosome
• Accession: NZ_CP060708
• Organism: Escherichia coli strain MFDpir

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: H9446_RS01180
• Protein ID: WP_001263489.1
• Coordinates: 252301..252699 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: H9446_RS01175
• Protein ID: WP_000554758.1
• Coordinates: 252005..252298 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H9446_RS01155	247637..248134	+	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-
H9446_RS01160	248358..250097	-	1740	WP_001334802.1	flagellar biosynthesis protein FlhA	-
H9446_RS01165	250042..250827	+	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-
H9446_RS01170	250898..251953	+	1056	WP_001226164.1	DNA polymerase IV	-
H9446_RS01175	252005..252298	+	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
H9446_RS01180	252301..252699	+	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
H9446_RS01185	252709..253161	+	453	WP_001059892.1	GNAT family N-acetyltransferase	-
H9446_RS01190	253479..253685	+	207	Protein_230	RtcB family protein	-
H9446_RS01195	253681..254202	+	522	Protein_231	peptide chain release factor H	-
H9446_RS01200	254259..255716	-	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
H9446_RS01205	255977..256435	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
-	257031..257111	+	81	NuclAT_12	-	-
-	257031..257111	+	81	NuclAT_12	-	-
-	257031..257111	+	81	NuclAT_12	-	-
-	257031..257111	+	81	NuclAT_12	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	gmhA/lpcA	221614..296245	74631

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T170765 WP_001263489.1 NZ_CP060708:252301-252699 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T170765 NZ_CP077699:c4395999-4395508 [Salmonella enterica subsp. enterica]
ATGATCTCCTCCCCTGAGCCGCTTCATGCCGGACATATTCTTACTCCGTTTTGCTGCGGTGTGGATTCTATGGATAACTG
GCTGAAACAGCGGGCGATGAAAAATCAGACCACTGGTGCGTCCCGTACCTTTGTGTGTTGTGGCAGCGATTCGAGCGTAC
TGGCCTATTACTCGCTGGCGTCCAGCGCGGTCACGACGAATACCGCCCCCGGTCGCTTTCGTCGCAATATGCCTGACCCG
ATTCCGATTGTGGTATTGGGGCGTCTGGCGGTGGATAAATCGCTTCATGGGCAGGGCGTTGGTCGGGCGCTGGTACGCGA
CGCAGGGCTGCGGGTTATTCAAGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCGC
GGGAATTTTACCAGCGGGTGGGGTTTGTACCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGGGGGATTTGGTG
GAGAGTGTTTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT170765 WP_000554758.1 NZ_CP060708:252005-252298 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT170765 NZ_CP077699:c4396289-4395996 [Salmonella enterica subsp. enterica]
ATGCCAGCCGCAAACAGTATGGCGATGAAACGCGAAACCCTGAACCTGCGTATCAAACCCGCCGAGCGCGATCTTATTGA
TCGAGCGGCGAAAGCCAGAGGGAAAAACCGCACTGACTTTGTGCTGGAAGCTGCCCGTGCTGCCGCCGAGGAAGCGCTGA
TCGAACAACGCATCATTATGGCCGATCCAGAGGCCTATCAGGAATTTCTGGCTCGTTTGGATCAAACACCTTCACCTAAT
GCCGCACTGCGTAAAACCATGCAGACCCCTGCGCCGTGGGAGCAGGAAAAATGA

Structures

Toxin

• AlphaFold DB: A0A090J8B1

Antitoxin

• AlphaFold DB: S1QAE3