Detailed information of TA system TA170705

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 382063..382817
• Replicon: chromosome
• Accession: NZ_CP060678
• Organism: Salmonella enterica strain 190610_1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: B5RFE2
• Locus tag: H9Q82_RS01815
• Protein ID: WP_000558168.1
• Coordinates: 382506..382817 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: H9Q82_RS01810
• Protein ID: WP_001259009.1
• Coordinates: 382063..382509 (-)
• Length: 149 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H9Q82_RS01780 (377239)	377239..378135	+	897	WP_001520529.1	sugar kinase	-
H9Q82_RS01785 (378169)	378169..378972	+	804	WP_000059693.1	DeoR/GlpR family DNA-binding transcription regulator	-
H9Q82_RS01790 (379163)	379163..379762	+	600	WP_000965695.1	glucose-1-phosphatase	-
H9Q82_RS01795 (379756)	379756..380628	+	873	WP_000921423.1	virulence factor BrkB family protein	-
H9Q82_RS01800 (380625)	380625..381062	+	438	WP_000560968.1	D-aminoacyl-tRNA deacylase	-
H9Q82_RS01805 (381107)	381107..382048	+	942	WP_001518251.1	fatty acid biosynthesis protein FabY	-
H9Q82_RS01810 (382063)	382063..382509	-	447	WP_001259009.1	type II toxin-antitoxin system HigA family antitoxin	Antitoxin
H9Q82_RS01815 (382506)	382506..382817	-	312	WP_000558168.1	type II toxin-antitoxin system HigB family toxin	Toxin
H9Q82_RS01820 (382903)	382903..383832	-	930	WP_001127705.1	alpha/beta hydrolase	-
H9Q82_RS01825 (384050)	384050..384361	+	312	WP_001159635.1	cytotoxic translational repressor of toxin-antitoxin stability system	-
H9Q82_RS01830 (384362)	384362..384652	+	291	WP_000362050.1	DNA-binding transcriptional regulator	-
H9Q82_RS01835 (384699)	384699..385628	-	930	WP_000027736.1	formate dehydrogenase accessory protein FdhE	-
H9Q82_RS01840 (385625)	385625..386260	-	636	WP_000829025.1	formate dehydrogenase cytochrome b556 subunit	-
H9Q82_RS01845 (386257)	386257..387159	-	903	WP_000331364.1	formate dehydrogenase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (93-146)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12324.26 Da
• Isoelectric Point: 9.3143

>T170705 WP_000558168.1 NZ_CP060678:c382817-382506 [Salmonella enterica]
VHVISRKPFNEAMLMYPNHELALTELLNVLEKKTLTQPEEMKRYIPSLDNFKHRDKWWVIDVSGNSLRLISYIDFRLHKI
FVKHIVSHAEYDKLTAYDRGNKE

Nucleotide

• Length: 312 bp

>T170705 NZ_CP077680:c1430361-1430143 [Salmonella enterica subsp. enterica serovar Kentucky]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTGTCCGACAATGAGCTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACGATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin

Protein

• Length: 149 a.a.
• Molecular weight: 16720.06 Da
• Isoelectric Point: 6.6451

>AT170705 WP_001259009.1 NZ_CP060678:c382509-382063 [Salmonella enterica]
MRTHRQMDATSAKKIVDTFSDAVKSVPLMGEDRNDNEYRRALALVEFLVDHDDLENPLFELLCARISEYEKHAPEFKALN
QHLEKTPPGVSVLRTLMDQYGLKAADLANELGSKSNVSNILNGRRALTVNHIKALTQRFKLPADAFIE

Nucleotide

• Length: 447 bp

>AT170705 NZ_CP077680:c1430763-1430389 [Salmonella enterica subsp. enterica serovar Kentucky]
ATGGACGAATACTCACCCAAAAGACATGATATAGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAATGACCCGACCTCAGCAGTCAACCTGCAGCTCAATGAGCTGATAGAGC
ATATTGCAACTTTCGCGCTTAATTATAAAATTAAGTACAATGAAGACAATAAATTGATTGCGCAGATAGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTATGGTATTAATACGCAGGATTTGCAAAAATGGCGTAAATCGGGCAACCGCCT
GTTCCGTTGTTTCGTCAACGCCACCAGGGCTAATCCTGTTAGTTTGTCTTGTTAA

Structures

Toxin

• AlphaFold DB: A0A1R2S541