Detailed information of TA system TA170566

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 156370..157124
• Replicon: chromosome
• Accession: NZ_CP060668
• Organism: Salmonella enterica strain 190819_2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: B5RFE2
• Locus tag: H9Q87_RS00745
• Protein ID: WP_000558168.1
• Coordinates: 156370..156681 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: H9Q87_RS00750
• Protein ID: WP_001259009.1
• Coordinates: 156678..157124 (+)
• Length: 149 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H9Q87_RS00715 (152028)	152028..152930	+	903	WP_000331364.1	formate dehydrogenase subunit beta	-
H9Q87_RS00720 (152927)	152927..153562	+	636	WP_000829025.1	formate dehydrogenase cytochrome b556 subunit	-
H9Q87_RS00725 (153559)	153559..154488	+	930	WP_000027736.1	formate dehydrogenase accessory protein FdhE	-
H9Q87_RS00730 (154535)	154535..154825	-	291	WP_000362050.1	DNA-binding transcriptional regulator	-
H9Q87_RS00735 (154826)	154826..155137	-	312	WP_001159635.1	cytotoxic translational repressor of toxin-antitoxin stability system	-
H9Q87_RS00740 (155355)	155355..156284	+	930	WP_001127705.1	alpha/beta hydrolase	-
H9Q87_RS00745 (156370)	156370..156681	+	312	WP_000558168.1	type II toxin-antitoxin system HigB family toxin	Toxin
H9Q87_RS00750 (156678)	156678..157124	+	447	WP_001259009.1	type II toxin-antitoxin system HigA family antitoxin	Antitoxin
H9Q87_RS00755 (157139)	157139..158080	-	942	WP_001518251.1	fatty acid biosynthesis protein FabY	-
H9Q87_RS00760 (158125)	158125..158562	-	438	WP_000560968.1	D-aminoacyl-tRNA deacylase	-
H9Q87_RS00765 (158559)	158559..159431	-	873	WP_000921423.1	virulence factor BrkB family protein	-
H9Q87_RS00770 (159425)	159425..160024	-	600	WP_000965695.1	glucose-1-phosphatase	-
H9Q87_RS00775 (160215)	160215..161018	-	804	WP_000059693.1	DeoR/GlpR family DNA-binding transcription regulator	-
H9Q87_RS00780 (161052)	161052..161948	-	897	WP_001520529.1	sugar kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (93-146)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12324.26 Da
• Isoelectric Point: 9.3143

>T170566 WP_000558168.1 NZ_CP060668:156370-156681 [Salmonella enterica]
VHVISRKPFNEAMLMYPNHELALTELLNVLEKKTLTQPEEMKRYIPSLDNFKHRDKWWVIDVSGNSLRLISYIDFRLHKI
FVKHIVSHAEYDKLTAYDRGNKE

Nucleotide

• Length: 312 bp

>T170566 NZ_CP077646:c2050378-2050205 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 149 a.a.
• Molecular weight: 16720.06 Da
• Isoelectric Point: 6.6451

>AT170566 WP_001259009.1 NZ_CP060668:156678-157124 [Salmonella enterica]
MRTHRQMDATSAKKIVDTFSDAVKSVPLMGEDRNDNEYRRALALVEFLVDHDDLENPLFELLCARISEYEKHAPEFKALN
QHLEKTPPGVSVLRTLMDQYGLKAADLANELGSKSNVSNILNGRRALTVNHIKALTQRFKLPADAFIE

Nucleotide

• Length: 447 bp

>AT170566 NZ_CP077646:c2050702-2050406 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: A0A1R2S541