Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 4132296..4133050 Replicon chromosome
Accession NZ_CP060666
Organism Salmonella enterica strain 190821_1

Toxin (Protein)


Gene name higB Uniprot ID B5RFE2
Locus tag H9Q88_RS20205 Protein ID WP_000558168.1
Coordinates 4132296..4132607 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag H9Q88_RS20210 Protein ID WP_001259009.1
Coordinates 4132604..4133050 (+) Length 149 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9Q88_RS20175 (4127954) 4127954..4128856 + 903 WP_000331364.1 formate dehydrogenase subunit beta -
H9Q88_RS20180 (4128853) 4128853..4129488 + 636 WP_000829025.1 formate dehydrogenase cytochrome b556 subunit -
H9Q88_RS20185 (4129485) 4129485..4130414 + 930 WP_000027736.1 formate dehydrogenase accessory protein FdhE -
H9Q88_RS20190 (4130461) 4130461..4130751 - 291 WP_000362050.1 DNA-binding transcriptional regulator -
H9Q88_RS20195 (4130752) 4130752..4131063 - 312 WP_001159635.1 cytotoxic translational repressor of toxin-antitoxin stability system -
H9Q88_RS20200 (4131281) 4131281..4132210 + 930 WP_001127705.1 alpha/beta hydrolase -
H9Q88_RS20205 (4132296) 4132296..4132607 + 312 WP_000558168.1 type II toxin-antitoxin system HigB family toxin Toxin
H9Q88_RS20210 (4132604) 4132604..4133050 + 447 WP_001259009.1 type II toxin-antitoxin system HigA family antitoxin Antitoxin
H9Q88_RS20215 (4133065) 4133065..4134006 - 942 WP_001518251.1 fatty acid biosynthesis protein FabY -
H9Q88_RS20220 (4134051) 4134051..4134488 - 438 WP_000560968.1 D-aminoacyl-tRNA deacylase -
H9Q88_RS20225 (4134485) 4134485..4135357 - 873 WP_000921423.1 virulence factor BrkB family protein -
H9Q88_RS20230 (4135351) 4135351..4135950 - 600 WP_000965695.1 glucose-1-phosphatase -
H9Q88_RS20235 (4136141) 4136141..4136944 - 804 WP_000059693.1 DeoR/GlpR family DNA-binding transcription regulator -
H9Q88_RS20240 (4136978) 4136978..4137874 - 897 WP_001520529.1 sugar kinase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(21-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(93-146)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12324.26 Da        Isoelectric Point: 9.3143

>T170560 WP_000558168.1 NZ_CP060666:4132296-4132607 [Salmonella enterica]
VHVISRKPFNEAMLMYPNHELALTELLNVLEKKTLTQPEEMKRYIPSLDNFKHRDKWWVIDVSGNSLRLISYIDFRLHKI
FVKHIVSHAEYDKLTAYDRGNKE

Download         Length: 312 bp

>T170560 NZ_CP077646:437330-437437 [Escherichia coli]
ATGACGCTCGCGAAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 149 a.a.        Molecular weight: 16720.06 Da        Isoelectric Point: 6.6451

>AT170560 WP_001259009.1 NZ_CP060666:4132604-4133050 [Salmonella enterica]
MRTHRQMDATSAKKIVDTFSDAVKSVPLMGEDRNDNEYRRALALVEFLVDHDDLENPLFELLCARISEYEKHAPEFKALN
QHLEKTPPGVSVLRTLMDQYGLKAADLANELGSKSNVSNILNGRRALTVNHIKALTQRFKLPADAFIE

Download         Length: 447 bp

>AT170560 NZ_CP077646:c437277-437216 [Escherichia coli]
TGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1R2S541

Antitoxin

Source ID Structure

References