Detailed information of TA system TA170525

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-couple_hipB
• Location: 3097008..3097662
• Replicon: chromosome
• Accession: NZ_CP060635
• Organism: Wansuia hejianensis strain NSJ-29

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: H9Q79_RS14315
• Protein ID: WP_118647195.1
• Coordinates: 3097300..3097662 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: H9Q79_RS14310
• Protein ID: WP_118647197.1
• Coordinates: 3097008..3097307 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H9Q79_RS14285 (H9Q79_14280)	3092747..3092992	+	246	WP_118647207.1	hypothetical protein	-
H9Q79_RS14290 (H9Q79_14285)	3093088..3093516	+	429	WP_118647205.1	hypothetical protein	-
H9Q79_RS14295 (H9Q79_14290)	3093531..3095345	+	1815	WP_249328581.1	hypothetical protein	-
H9Q79_RS14300 (H9Q79_14295)	3095342..3096280	+	939	WP_249328582.1	hypothetical protein	-
H9Q79_RS14305 (H9Q79_14300)	3096354..3096737	-	384	WP_249328583.1	hypothetical protein	-
H9Q79_RS14310 (H9Q79_14305)	3097008..3097307	-	300	WP_118647197.1	helix-turn-helix transcriptional regulator	Antitoxin
H9Q79_RS14315 (H9Q79_14310)	3097300..3097662	-	363	WP_118647195.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H9Q79_RS14320 (H9Q79_14315)	3097830..3099245	+	1416	WP_249328584.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3071520..3121859	50339

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (35-109)

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-91)

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 14259.45 Da
• Isoelectric Point: 9.7934

>T170525 WP_118647195.1 NZ_CP060635:c3097662-3097300 [Wansuia hejianensis]
MHQIYFYRDKNGKEPVADYLAELASKKDKDSRIKLNKIRDYVKTLSEYGTQVGEPYIKHLDGEIWELRPLRDRILFVGWV
NGSYVLLHQFMKKTQKTPAREIEKAKRELADLIERGVKYE

Nucleotide

• Length: 363 bp

>T170525 NZ_CP077423:649625-649894 [Streptococcus gallolyticus subsp. gallolyticus TX20005]
ATGACTTATAGATTGGTTGTTAGTGACAAGGTCAAGAAACAACTAAAGAAAATGGATAAGCATGTAAGGCTTATGCTTGC
TAAAGACATGAAGAAACACCTTGATGGTTTAGAGAACCCTAGACAAATTGGAAAAGCACTAATAGGTCAATTCAAAGGCC
TATGGCGTTACCGAATAGGAAATTATAGAGTTATTTGTGATATTATGGACGATGAATTAGTTATTCTTGCTATTGAAATC
GGACATAGAAAAGATATTTATAAAAACTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11008.75 Da
• Isoelectric Point: 6.3863

>AT170525 WP_118647197.1 NZ_CP060635:c3097307-3097008 [Wansuia hejianensis]
MSNTNNSAIGRSWEEVERELFTPEEIAESNLRVALIGELIKARQEKGISQKRLEEMSGVKQPIIARMEKGNTNPQLETVL
KILAPLGKTLAIVPLDPTK

Nucleotide

• Length: 300 bp

>AT170525 NZ_CP077423:649408-649635 [Streptococcus gallolyticus subsp. gallolyticus TX20005]
ATGTCAATCGTATCACTTAGACTAAATGAAAAAGAAGAAGAACTTTTTAGAAGTTATTCTATCCATACAGGAAAGACATT
ATCAGAATTGTTTAAAACAGCTTTAGCTGAACAAATTGAAAATCAACTAGATTATGAAACAGGTATCCAAGCATTGAAAG
AATTCAAAAAGAATCCTGTTACTCACTCTATTGATGATATTATTGAGGAGTTTGAAAATGACTTATAG