Detailed information of TA system TA170489

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 374051..374574
• Replicon: chromosome
• Accession: NZ_CP060584
• Organism: Staphylococcus aureus strain WH52

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: X5DYX7
• Locus tag: H9M95_RS01655
• Protein ID: WP_000074048.1
• Coordinates: 374308..374574 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: W8TQT5
• Locus tag: H9M95_RS01650
• Protein ID: WP_000587616.1
• Coordinates: 374051..374308 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H9M95_RS01630	369131..370324	+	1194	WP_000675401.1	MFS transporter	-
H9M95_RS01635	370548..371297	-	750	WP_000072443.1	hypothetical protein	-
H9M95_RS01640	371492..373069	+	1578	WP_194251061.1	FMN-binding glutamate synthase family protein	-
H9M95_RS01645	373237..373692	-	456	WP_000732082.1	DUF1307 domain-containing protein	-
H9M95_RS01650	374051..374308	+	258	WP_000587616.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
H9M95_RS01655	374308..374574	+	267	WP_000074048.1	Txe/YoeB family addiction module toxin	Toxin
H9M95_RS01660	374929..376005	+	1077	WP_001076671.1	M42 family metallopeptidase	-
H9M95_RS01665	376252..376914	+	663	WP_000923520.1	ATP-binding cassette domain-containing protein	-
H9M95_RS01670	376907..377683	+	777	WP_000072147.1	iron export ABC transporter permease subunit FetB	-
H9M95_RS01675	377872..378576	+	705	WP_048665789.1	helix-turn-helix domain-containing protein	-
H9M95_RS01680	378495..379424	+	930	WP_117206203.1	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (25-87)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-68)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10648.23 Da
• Isoelectric Point: 10.1384

>T170489 WP_000074048.1 NZ_CP060584:374308-374574 [Staphylococcus aureus]
MSNYTVKIKNSAKSDLKKIKHSYLKKSFLEIVETLKNDPYKITQSFEKLEPKYLERYSRRINHQHRVVYTVDDRNKEVLI
LSAWSHYD

Nucleotide

• Length: 267 bp

>T170489 NZ_CP077407:c4715137-4714664 [Enterobacter roggenkampii]
ATGATTTTTTATCGTCTGGTGACAGGACGCTATGCCAGCGAGGCGTGGAGTGGGAGTGGTGCAAACCAGTATGGCGGACG
GTGGAATCATAAAGGCCATCCAGCTGTGTATGTCTCCACCTCCATCTCGCTGGCCTCACTGGAGATTTTGGTACATGTCA
GAAAAGATACCGTGCTGAATCAGTACCAGCTTTTCAGCATTGATATCCCGGATGACCAGATAGATTACCTGGATAAAGCG
TGGCTCCCCGAGGACTGGCAGGAAAATCCGGCGCCGGTATCGACAATGGATCTGGGAACCGGTTGGTTGCAGGCTAACAG
TGCCCTCGCGCTTATCTTACCTTCCTGTATCATTCCTTATGAAAACAATGCCATCCTTAACCCCTTACATCCGGCGTTCC
ACTCGGCGCTCAATTCAGTTCAGCAACTCCCCTTTATTTTTGATACCCGCCTGGCTGAAAAAACCGCACCCTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9774.73 Da
• Isoelectric Point: 4.1353

>AT170489 WP_000587616.1 NZ_CP060584:374051-374308 [Staphylococcus aureus]
MIITSPTEARKDFYQLLKNVNNNHEPIYISGNNAENNAVIIGLEDWKSIQETIYLESTGTMDKVREREKDNSGTTNIDDI
DWDNL

Nucleotide

• Length: 258 bp

>AT170489 NZ_CP077407:c4715577-4715134 [Enterobacter roggenkampii]
ATGAGAACATGGATCCCCGCACAGAAACCTGCTGATAACGCACTTTGGCGTTACGCCGGTTTACCCGCCAACCGAGGTAT
GCGGTTGATTGAATTTCTCAATCAGGGATTACCCGTCAGTGTGCTCGACAACATCCACGAGTGGACGTCCATGTCGAAAG
CGGACATTTTGCGTGTCACGGGTATCAACGAACGTAACGTGGCCCGCCGCAAAAGCGCGGGACGGACGTTAACGCCCGGC
GAGAGCGAACGCGTGGCCCGTTTCGTTCGGGTGCTGGATGCTGCCGTTGACTATTTTGGCAGTAAAGAAGAAGCATGGAC
CTGGCTGCAGGCACCGGTGCGCGGACTGGGGAACGTGGCGCCCGTCGACTTAATAGCGACGGAAACCGGTGCGCTGGAAG
TCACCGATTTGATTGGTCGCCTGGAACACGGCGTGTTCGCATGA

Structures

Toxin

• PDB: 7V6W
• PDB: 7V5Y
• PDB: 7V5Z

Antitoxin

• PDB: 7V6W
• PDB: 7V5Z
• PDB: 7V5Y
• AlphaFold DB: W8TQT5