Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3085836..3086499 Replicon chromosome
Accession NZ_CP060441
Organism Citrobacter cronae strain S175-1

Toxin (Protein)


Gene name higB Uniprot ID A0A2Z3X092
Locus tag H3W50_RS15145 Protein ID WP_038636359.1
Coordinates 3086197..3086499 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A2Z3X2H4
Locus tag H3W50_RS15140 Protein ID WP_038636361.1
Coordinates 3085836..3086177 (-) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H3W50_RS15110 (3081360) 3081360..3082118 + 759 WP_038636380.1 phosphonate C-P lyase system protein PhnK -
H3W50_RS15115 (3082226) 3082226..3082906 + 681 WP_038636377.1 phosphonate C-P lyase system protein PhnL -
H3W50_RS15120 (3082903) 3082903..3084039 + 1137 WP_038636374.1 alpha-D-ribose 1-methylphosphonate 5-triphosphate diphosphatase -
H3W50_RS15125 (3084042) 3084042..3084596 + 555 WP_038636371.1 ribose 1,5-bisphosphokinase -
H3W50_RS15130 (3084583) 3084583..3085017 + 435 WP_038636367.1 aminoalkylphosphonate N-acetyltransferase -
H3W50_RS15135 (3085026) 3085026..3085784 + 759 WP_038636364.1 phosphonate metabolism protein PhnP -
H3W50_RS15140 (3085836) 3085836..3086177 - 342 WP_038636361.1 HigA family addiction module antitoxin Antitoxin
H3W50_RS15145 (3086197) 3086197..3086499 - 303 WP_038636359.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
H3W50_RS15150 (3086655) 3086655..3086984 - 330 WP_038636356.1 DDRRRQL repeat protein YjdP -
H3W50_RS15155 (3087054) 3087054..3089318 - 2265 WP_038636353.1 hybrid sensor histidine kinase/response regulator -
H3W50_RS15160 (3089433) 3089433..3090956 + 1524 WP_038636350.1 sugar ABC transporter ATP-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Cro/C1-type helix-turn-helix domain

(23-70)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11856.50 Da        Isoelectric Point: 9.9581

>T170410 WP_038636359.1 NZ_CP060441:c3086499-3086197 [Citrobacter cronae]
MSKSLNIRNFRDTWLEDFFERATPHRKIPADIHTALARKLDIINAAVSHRDLRSPPGNRYEELTGKLQEYSSIRVNKQYR
LIFKWVNGKAEDVYLDPHIY

Download         Length: 303 bp

>T170410 NZ_CP077332:c40646-40269 [Lelliottia amnigena]
ATGGCCGACAAAGAAAAACCGCTCGAATGGATCGCGAGCAGCTACAGGGATCTGATGGCGTTGTCGCCCGATGTGCGACG
GCGTTTCGGTTACGCGCTGTCGCTGGCCCAGATGGGCGACCAGGACGACGCGGCCAAGGTGCTCAAGGGCTTCGGCGGGG
CTGGCGTGCTGGAGGTCGTCGAAGACGATGCCGGCGGCACGTACCGGGCGGTCTACACGGTCAAGTTTGCGGAAGCGGTG
TTCGTCCTGCACTGCTTCCAGAAGAAGAGCAAGAGCGGGATCGCCACGCCGAAGGTGGACATGGACATCATCCGCGCTCG
GCTGAAGGTGGCCGAGGCAATGGCACAGGAGCTACGAAATGCAAAAACGAATCATTGA

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13063.93 Da        Isoelectric Point: 7.2038

>AT170410 WP_038636361.1 NZ_CP060441:c3086177-3085836 [Citrobacter cronae]
MKQATRKPTTVGDVLQYEYLEPLDLKINDLAEILHVHRNTVSALVNNNRKLTTDMAFRLAKAFDTSVDFWRNLQSAVDLW
EVENDMRAQEELNRIMSVKDFVAQRNVATKKVA

Download         Length: 342 bp

>AT170410 NZ_CP077332:c40288-39953 [Lelliottia amnigena]
ATGCAAAAACGAATCATTGAAGGCGTCGAGGCTCAACGAAGCTCGGGCAACGTCTTCGCCGACCTGGGGCTGCCCGATGC
CGAAAAGCTGAAGATCAAGACCGGCCTGGTCATCGAGATCAGGAAGGCGATGCGCGCCCTTGACCTGACGCAACAAGCGG
CGGCCAAGCGTATGGGCATCCCGCAACCGAAGGTATCGGGCATGATGCACGGCGACTTCACCAACCTGTCCGAGCGCAAG
CTGATGGACTGCCTGAACCGCCTCGGCTACGACATCGAAATCAAGGTGCGGCCGGCAACCGAGCCGGTCGGCCACCTGAC
GCTTGCAACCGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2Z3X092

Antitoxin

Source ID Structure
AlphaFold DB A0A2Z3X2H4

References