Detailed information of TA system TA170393

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 3789390..3790043
• Replicon: chromosome
• Accession: NZ_CP060440
• Organism: Serratia marcescens strain M158-1-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: H3V66_RS18445
• Protein ID: WP_038878324.1
• Coordinates: 3789693..3790043 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A5C7DBM3
• Locus tag: H3V66_RS18440
• Protein ID: WP_019453270.1
• Coordinates: 3789390..3789689 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H3V66_RS18415	3784810..3785733	-	924	WP_240023217.1	3-hydroxyacyl-CoA dehydrogenase family protein	-
H3V66_RS18420	3785752..3786936	-	1185	WP_060451848.1	acetyl-CoA C-acetyltransferase	-
H3V66_RS18425	3786951..3787604	-	654	WP_240023218.1	CoA transferase subunit B	-
H3V66_RS18430	3787616..3788314	-	699	WP_004933280.1	CoA transferase subunit A	-
H3V66_RS18435	3788483..3789400	+	918	WP_063991524.1	LysR family transcriptional regulator	-
H3V66_RS18440	3789390..3789689	-	300	WP_019453270.1	XRE family transcriptional regulator	Antitoxin
H3V66_RS18445	3789693..3790043	-	351	WP_038878324.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H3V66_RS18450	3790315..3791319	+	1005	WP_174136431.1	hypothetical protein	-
H3V66_RS18455	3791323..3791715	+	393	WP_038878318.1	hypothetical protein	-
H3V66_RS18460	3791729..3792124	+	396	WP_240023219.1	hypothetical protein	-
H3V66_RS18465	3792127..3792552	+	426	WP_049272484.1	hypothetical protein	-
H3V66_RS18470	3792549..3792848	-	300	WP_077791580.1	hypothetical protein	-
H3V66_RS18475	3793094..3794464	+	1371	WP_240023220.1	glutamine synthetase family protein	-
H3V66_RS18480	3794494..3794904	+	411	WP_240023221.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-83)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13574.83 Da
• Isoelectric Point: 8.2712

>T170393 WP_038878324.1 NZ_CP060440:c3790043-3789693 [Serratia marcescens]
MWTVMTTEEFDRWLCEQDESTQEKVLAALVMLERAGPSLRRPFVDVLRGSLHPNMKELRIQHKGRPIRAFFAFDPARQAI
VLCAGDKTGNEKRFYKVMLPIADAQFTQYLMCYFKE

Nucleotide

• Length: 351 bp

>T170393 NZ_CP077318:46917-47183 [Providencia rettgeri]
TTGGTCTGGACGATTAGTTATTCTGAACGGGCGTTAAAGTCTCTACGAAAAATGGATAAACAAAACGCCCGTCGCATTGT
CGATTTTATGGATTTACGCATTGCCGCTTTAGATGATCCTCGCTTGTCAGGCAAACCATTAAAAGGTGAATTGGGTGAGT
TCTGGCGTTATAGAGTCGGTGACTATCGGATACTATGTGAGATAAGGGATGATGAACTTGTTATTCTTGCTGCTACTATC
GGTCACCGTAAAGAAGTTTATGATTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11013.71 Da
• Isoelectric Point: 7.3207

>AT170393 WP_019453270.1 NZ_CP060440:c3789689-3789390 [Serratia marcescens]
MSTLKQLLASRTPESRRRIEEMAEEMLFEAHLHQLREALNISQQELAKTLGISQPSVAAIEGRGNELKISTLKRYVEAMG
GTLRLDVELPGGKHVGFTV

Nucleotide

• Length: 300 bp

>AT170393 NZ_CP077318:46709-46933 [Providencia rettgeri]
ATGTTAGCTATTCGATTATCTGATGACATTGAAAGTCGTCTTGAGTCACTCGCAAAACTGACAGGTAGAACCAAAACATT
CTATGCGAGAGAGGCAATTGTTACCCACTTGGAAGACTTAGAAGACTACTATTTATCCGCTGAAACCGTTGCAAAAATTC
GCCGCGGTGAAGAATTGGTTCATTCGTCTGAAGACGTGAGGAAAACACTTGGTCTGGACGATTAG

Structures

Antitoxin

• AlphaFold DB: A0A5C7DBM3