Detailed information of TA system TA170380

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 1103434..1104091
• Replicon: chromosome
• Accession: NZ_CP060440
• Organism: Serratia marcescens strain M158-1-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: H3V66_RS05180
• Protein ID: WP_174135352.1
• Coordinates: 1103434..1103757 (+)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A0M5M3W3
• Locus tag: H3V66_RS05185
• Protein ID: WP_060420390.1
• Coordinates: 1103795..1104091 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H3V66_RS05155	1099311..1100069	+	759	WP_174135358.1	2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase	-
H3V66_RS05160	1100071..1100949	-	879	WP_049273981.1	EamA family transporter RarD	-
H3V66_RS05165	1101089..1101736	+	648	WP_240023798.1	DNA oxidative demethylase AlkB	-
H3V66_RS05170	1101719..1102654	-	936	WP_174135354.1	LysR family transcriptional regulator	-
H3V66_RS05175	1102740..1103369	+	630	WP_240024355.1	alanyl-tRNA editing protein	-
H3V66_RS05180	1103434..1103757	+	324	WP_174135352.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H3V66_RS05185	1103795..1104091	+	297	WP_060420390.1	NadS family protein	Antitoxin
H3V66_RS05190	1104099..1104341	-	243	WP_240023799.1	hypothetical protein	-
H3V66_RS05195	1104646..1105554	+	909	WP_240023800.1	sugar glycosyltransferase	-
H3V66_RS05200	1105532..1106800	+	1269	WP_240023801.1	O-antigen ligase family protein	-
H3V66_RS05205	1106758..1107393	-	636	WP_126179216.1	flavodoxin family protein	-
H3V66_RS05210	1107509..1108405	+	897	WP_240023802.1	LysR substrate-binding domain-containing protein	-
H3V66_RS05215	1108475..1109062	+	588	WP_033635168.1	nitroreductase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (45-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-83)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 12301.04 Da
• Isoelectric Point: 8.0267

>T170380 WP_174135352.1 NZ_CP060440:1103434-1103757 [Serratia marcescens]
MDYLEFIETTVFSRKRAELMKDDDFQAFQSYLLLNHHEGDTISHTGGCKKIRWGLDGKGKRGGVRVIYYLTAPSGRVYLL
LIYSKNEKDDLSGQEKAILRSIVQQLD

Nucleotide

• Length: 324 bp

>T170380 NZ_CP060440:1103434-1103757 [Serratia marcescens]
ATGGACTATCTGGAGTTTATCGAAACGACCGTTTTCAGCCGAAAGCGGGCTGAATTGATGAAGGATGATGACTTTCAGGC
TTTCCAAAGCTATTTGCTGTTGAACCATCATGAAGGGGATACGATCAGCCACACGGGCGGATGCAAAAAGATACGCTGGG
GGCTGGACGGAAAGGGAAAACGCGGCGGCGTAAGAGTGATTTACTATCTGACGGCGCCCAGCGGCCGAGTCTATTTATTG
TTGATATACAGTAAAAATGAAAAGGATGATTTATCCGGACAGGAAAAGGCGATATTGAGATCCATCGTTCAACAACTTGA
TTAA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11003.00 Da
• Isoelectric Point: 10.1906

>AT170380 WP_060420390.1 NZ_CP060440:1103795-1104091 [Serratia marcescens]
MKKELFDDLVSSMNEMVAIEKGKMTPKSEHVHRHAIPDVKLLRKSRGMKQAEFAKAVGASVGLVQSWEQERRIPSGSALK
LLLLIERNPALLDELLVI

Nucleotide

• Length: 297 bp

>AT170380 NZ_CP060440:1103795-1104091 [Serratia marcescens]
ATGAAAAAAGAACTTTTCGACGATTTGGTCAGCAGTATGAACGAAATGGTGGCGATCGAAAAGGGTAAGATGACGCCAAA
ATCCGAGCATGTTCACCGTCATGCTATTCCAGACGTCAAGCTGCTGCGCAAATCCCGTGGGATGAAGCAGGCGGAGTTTG
CCAAGGCTGTGGGGGCCAGCGTGGGACTGGTGCAAAGCTGGGAGCAGGAACGCCGCATACCCTCGGGGTCGGCACTGAAA
CTTCTGCTGCTGATTGAGCGTAACCCGGCGTTGCTCGATGAGTTGCTGGTGATTTAA

Structures

Antitoxin

• AlphaFold DB: A0A0M5M3W3