Detailed information of TA system TA170376

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 293461..294113
• Replicon: chromosome
• Accession: NZ_CP060440
• Organism: Serratia marcescens strain M158-1-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: H3V66_RS01380
• Protein ID: WP_004931830.1
• Coordinates: 293769..294113 (-)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A086GAN0
• Locus tag: H3V66_RS01375
• Protein ID: WP_004931828.1
• Coordinates: 293461..293763 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H3V66_RS01350	288469..289116	-	648	WP_038878652.1	DsbA family protein	-
H3V66_RS01355	289185..290069	-	885	WP_033635778.1	MBL fold metallo-hydrolase	-
H3V66_RS01360	290178..291086	+	909	WP_015378928.1	LysR family transcriptional regulator	-
H3V66_RS01365	291114..292037	-	924	WP_240023579.1	LysR family transcriptional regulator	-
H3V66_RS01370	292171..293433	+	1263	WP_240023581.1	diaminopimelate decarboxylase	-
H3V66_RS01375	293461..293763	-	303	WP_004931828.1	XRE family transcriptional regulator	Antitoxin
H3V66_RS01380	293769..294113	-	345	WP_004931830.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H3V66_RS01385	294273..295292	-	1020	WP_049202159.1	HTH-type transcriptional regulator GalR	-
H3V66_RS01390	295513..297771	+	2259	WP_240023583.1	molybdopterin guanine dinucleotide-containing S/N-oxide reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-104)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-85)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13383.44 Da
• Isoelectric Point: 10.6087

>T170376 WP_004931830.1 NZ_CP060440:c294113-293769 [Serratia marcescens]
MWQVVTVERFDDWFLALNNAQQTSILAAIYKLQTFGPQLARPHADTLHFSDAVRQLKELRIQHRGRPFRVFFAFDPQRQA
VLLCGGDKTGDKRFYQRMLPIAAMEFSHYLATRR

Nucleotide

• Length: 345 bp

>T170376 NZ_CP077306:5607-5897 [Acinetobacter pittii]
ATGACACTGACGATTAGGTACAAGAAACAATTTAAAAAAGATTTTAAACGGGTAAGCTCAAACCCAAAGCTTAAGAAAGG
ACTCTTAATTTTGAAGGATCTTATTGATGAGCATTTGGTAAAAACAGGGACTGTTCCAGATGAGTATTTACCTCATCCTT
TGATTGGTGAGTGGAAACCCCATATGGAATGCCATATTTTACCTGATTTATTATTAATTTGGGATGTGGATGAAGATAAT
AATGAGCTAATACTTGCTCGGATTGGATCTCATTCTGAACTTTTTGGATAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11080.97 Da
• Isoelectric Point: 10.1998

>AT170376 WP_004931828.1 NZ_CP060440:c293763-293461 [Serratia marcescens]
MAKPLSQLIDKLDPAVVAAARQKADQEIFELRLAMLREELAVSQVELAKRLGISQPSVANLEKRGSEIKLSSLKRYIEAM
GGTLSLDVQLPNGQHRRMTL

Nucleotide

• Length: 303 bp

>AT170376 NZ_CP077306:5348-5623 [Acinetobacter pittii]
ATGAATACTTTGGCAAAAAAAGATGAGTATGTACGTGCTAGGGTTCCAAATGAATTAAAGGTTCAAGCGGAGCGAATTCT
AAAAGAAGTGGGTTTAAATACTACAGATGCTATTCGCTTATTATTAACGCAAGTAGTAAATCGAGGTGGATTTCCTTTAG
AGTTAAGAACCCCTAACCGTACAACAATTGAGGCTATGAACTCTGTTGCAGAACCTAAAAAATTTAGTTCTGCTCAGGAA
TTGTTTACGGATTTAGAAGATGACACTGACGATTAG

Structures

Antitoxin

• AlphaFold DB: A0A086GAN0