Detailed information of TA system TA170337

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3447530..3448257
• Replicon: chromosome
• Accession: NZ_CP060401
• Organism: Xenorhabdus nematophila strain SII

Toxin (Protein)

• Gene name: higB
• Uniprot ID: D3VFW9
• Locus tag: H8F46_RS15400
• Protein ID: WP_013185754.1
• Coordinates: 3447530..3447841 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: H8F46_RS15405
• Protein ID: WP_013185753.1
• Coordinates: 3447838..3448257 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H8F46_RS15385	3443295..3443582	-	288	WP_010846183.1	1,2-phenylacetyl-CoA epoxidase subunit B	-
H8F46_RS15390	3443653..3444594	-	942	WP_010846184.1	1,2-phenylacetyl-CoA epoxidase subunit A	-
H8F46_RS15395	3444923..3446995	+	2073	WP_038219368.1	phenylacetic acid degradation bifunctional protein PaaZ	-
H8F46_RS15400	3447530..3447841	+	312	WP_013185754.1	type II toxin-antitoxin system HigB family toxin	Toxin
H8F46_RS15405	3447838..3448257	+	420	WP_013185753.1	helix-turn-helix domain-containing protein	Antitoxin
H8F46_RS15410	3448357..3448524	+	168	WP_038219367.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
H8F46_RS15415	3448566..3449885	+	1320	Protein_2996	IS701 family transposase	-
H8F46_RS15420	3450079..3450408	+	330	WP_010846191.1	universal stress protein UspB	-
H8F46_RS15425	3450534..3452024	-	1491	WP_013185749.1	inorganic phosphate transporter PitA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (84-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12535.52 Da
• Isoelectric Point: 10.0558

>T170337 WP_013185754.1 NZ_CP060401:3447530-3447841 [Xenorhabdus nematophila]
MHVISKEPFEQAVRDYPNDANVLITTYKLLKEKNFDSPLELQKLFPSLDNFKHRNKWWVIDIGGNNLRMIAYINFMNKRF
YVKHIVTHKEYDKLTKKYRETKE

Nucleotide

• Length: 312 bp

>T170337 NZ_CP077291:c1781409-1781077 [Citrobacter farmeri]
ATGGACAGAGGGGAAATCTGGCTCGTCTCACTGGATCCTACAGCCGGACATGAACAAAGCGGAAAACGTCCGGTACTTAT
CGTTTCTCTGGCATCGTTTAACATGCTGACCCAGCTACCCGTTGTTGTTCCCGTCACCAGCGGCGGAAACTTCGCCCGCT
CAGCCGGTTTTACCGTCTCACTGGACGGCGCTGGAACAAAAACAACTGGCGTCATTCGCTGCGACCAGCCCAGAACCATT
GATATGGGGGCCCGAAATGGTAAGCGGCTGGAACGTATACCCGACGCTGTCGTGAATGAGGTGCTGGCGCGACTGGAAGC
CATCCTGATCTGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15251.61 Da
• Isoelectric Point: 5.6660

>AT170337 WP_013185753.1 NZ_CP060401:3447838-3448257 [Xenorhabdus nematophila]
MIREPIKALQATTALIAAVPFLGSSASEKDYRDALAMVDYLIENHDESPLINILAAKIAEYEDSNKRFAAFNKAVEVMPT
GVAALRVLMDQHGLSYSDLQEEIGSKSLISQIFSGKRALTIAHIKALSARFDVKPDIFL

Nucleotide

• Length: 420 bp

>AT170337 NZ_CP077291:c1781667-1781410 [Citrobacter farmeri]
ATGTATACCACACGTCTGAAAAAGGTCGGCGGATCCGTCATGCTGGCGGTTCCTCCTGCTGTGCTGAAAACGCTGGCGCT
GTCGACAGACAGTGAAGTGGGCATGACCATTGATAATGGCTGCCTTATTATTGAACCCCAGAAACGGCCCCGTTATTCGC
TTGAGGAACTGCTGGCGCAGTGCGATCCGCACGCCGAAATGAGCGAAGAGGATCGGGAATGGATTAATGCGCCTGCTGTG
GGCAAGGAGATCCTGTAA

Structures

Toxin

• AlphaFold DB: D3VFW9