Detailed information of TA system TA170300

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3227921..3228485
• Replicon: chromosome
• Accession: NZ_CP060396
• Organism: Synechococcus sp. CBW1006

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: H8F26_RS15890
• Protein ID: WP_197155667.1
• Coordinates: 3228207..3228485 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: H8F26_RS15885
• Protein ID: WP_197155661.1
• Coordinates: 3227921..3228202 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H8F26_RS15860	3223684..3224232	-	549	WP_197155642.1	restriction endonuclease subunit S	-
H8F26_RS15865	3224281..3225315	+	1035	WP_197155644.1	IS5 family transposase	-
H8F26_RS15870	3225752..3227215	-	1464	WP_197155649.1	N-6 DNA methylase	-
H8F26_RS15875	3227212..3227598	-	387	WP_197155650.1	type II toxin-antitoxin system VapC family toxin	-
H8F26_RS15880	3227622..3227882	-	261	WP_197155658.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
H8F26_RS15885	3227921..3228202	-	282	WP_197155661.1	HigA family addiction module antidote protein	Antitoxin
H8F26_RS15890	3228207..3228485	-	279	WP_197155667.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H8F26_RS15895	3228556..3231912	-	3357	WP_197155672.1	type I restriction-modification system endonuclease	-
H8F26_RS15900	3231909..3232640	-	732	WP_197155678.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (3-78)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10532.16 Da
• Isoelectric Point: 8.1501

>T170300 WP_197155667.1 NZ_CP060396:c3228485-3228207 [Synechococcus sp. CBW1006]
VIRSFRCRDTLALFEGRPVRRFGSIARVACRKLAILDAAGCLNDLMIPPGNRLEPLRGDRHGQHSIRINDQFRLCFVWTE
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T170300 NZ_CP077264:c1606195-1605674 [Enterobacter hormaechei]
GTGGCAAATCTCACGATCGAAATGTTATCAGAGGGAACCGATTACGATTTCGGCGATTTCGATTGTGGTGAACCCTCGCT
AAATGCCTTCCTTACCGAGCACCTGGTACGTCAGCACGGCGGGCGTATTTTACGCGGCTACCTGCTTAAAGAACGCGACC
ATCCGCGTGTCCTGGGCTATTACACACTCTCGGGAAGTTGCTTTGAAAAAGCGATGCTTCCTTCAAAGACTCAGCAGCGC
CGCATCCCATACAGCAACGTCCCCAGCGTGACGCTGGGGCGTCTGGCTGTCCATAAAGAACTCCAGGGAAACGAGTGGGG
AACAACGCTTGTCACACACGCTATGCGCGTTGTTTATCTGGCGTCGCAGGCTGTTGGCGTCCACGGAATTTTTGTGGATG
CGCTTAACGAACGGGCCAAACGATTTTATCTGAAGCTGGGGTTTATCCCGTTGGCAGGTGAAAACAGCAGTTCGCTGTTC
TTCCCCACGCAATCTATCGAACGACTGTTTGAGCAGTCGTGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10310.18 Da
• Isoelectric Point: 9.2008

>AT170300 WP_197155661.1 NZ_CP060396:c3228202-3227921 [Synechococcus sp. CBW1006]
MLVPPGEVLLEEFLRPMGVSQYRLALAIGVPESRINAIVKGRRAITADTDLRLCRFFGLSEGFWLRMQASHDLKLAKQAL
ASVLPAIEPLQHA

Nucleotide

• Length: 282 bp

>AT170300 NZ_CP077264:c1606471-1606202 [Enterobacter hormaechei]
ATGCCAGCACTCAAAAAACAGCGTATCGATCTTCGCCTGTCGGACGAAGACAAAACAATGATTGAAGAAGCGGCAGCGAT
GACCAACCAAACGATCACCCAGTTTATGGTGAACAGTGCGTCAGAGCGTGCTGCCGAAGTCATCGAACAGCATCGGCGTC
TCGTATTAAACGAAGCCTCATGGATTGCCGTTATGGATGCCATCGACAATCCGCCAGAGCCAAATGAACGCCTGAAGCGA
GCGGCGAAACGTCTTCGAAACATGGAGTGA