Detailed information of TA system TA170269

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 5851586..5852181
• Replicon: chromosome
• Accession: NZ_CP060392
• Organism: Pseudomonas aeruginosa strain 1903031130

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: H8A93_RS27340
• Protein ID: WP_003113526.1
• Coordinates: 5851903..5852181 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: H8A93_RS27335
• Protein ID: WP_003133769.1
• Coordinates: 5851586..5851891 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H8A93_RS27320	5848292..5849155	-	864	WP_031761182.1	integrase domain-containing protein	-
H8A93_RS27325	5849756..5850913	-	1158	WP_031761183.1	hypothetical protein	-
H8A93_RS27335	5851586..5851891	-	306	WP_003133769.1	HigA family addiction module antidote protein	Antitoxin
H8A93_RS27340	5851903..5852181	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H8A93_RS27345	5852510..5854738	+	2229	WP_023104079.1	TonB-dependent receptor	-
H8A93_RS27350	5854809..5855456	-	648	WP_003095021.1	carbonate dehydratase	-
H8A93_RS27355	5855518..5856756	-	1239	WP_003095023.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (6-82)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T170269 WP_003113526.1 NZ_CP060392:c5852181-5851903 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T170269 NZ_CP077242:2375940-2376227 [Escherichia fergusonii]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCCAAAAGTAATCGGGTGAAACATGACATCCGCTTTGAAGATGCGGT
GTTAGTGTTTGATGATCCACAACATTTGTCACAGCAAGATCGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11214.73 Da
• Isoelectric Point: 4.6081

>AT170269 WP_003133769.1 NZ_CP060392:c5851891-5851586 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGEQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT170269 NZ_CP077242:2376214-2376516 [Escherichia fergusonii]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCAGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Structures

Toxin

• AlphaFold DB: V6ALY3