Detailed information of TA system TA170190

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4531028..4531758
• Replicon: chromosome
• Accession: NZ_CP060276
• Organism: Serratia ureilytica strain CC119

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A8B4GR63
• Locus tag: D0895_RS21405
• Protein ID: WP_004937033.1
• Coordinates: 4531028..4531339 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: D0895_RS21410
• Protein ID: WP_089192287.1
• Coordinates: 4531336..4531758 (+)
• Length: 141 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D0895_RS21375	4526343..4527068	+	726	WP_004937040.1	LPS export ABC transporter ATP-binding protein	-
D0895_RS21380	4527121..4528554	+	1434	WP_004937039.1	RNA polymerase factor sigma-54	-
D0895_RS21385	4528578..4528865	+	288	WP_004937038.1	ribosome hibernation promoting factor	-
D0895_RS21390	4529021..4529482	+	462	WP_016930212.1	PTS IIA-like nitrogen regulatory protein PtsN	-
D0895_RS21395	4529610..4530464	+	855	WP_015379177.1	RNase adapter RapZ	-
D0895_RS21400	4530461..4530733	+	273	WP_004937034.1	PTS phosphocarrier protein NPr	-
D0895_RS21405	4531028..4531339	+	312	WP_004937033.1	type II toxin-antitoxin system HigB family toxin	Toxin
D0895_RS21410	4531336..4531758	+	423	WP_089192287.1	helix-turn-helix domain-containing protein	Antitoxin
D0895_RS21415	4531765..4533681	-	1917	WP_033644486.1	BglG family transcription antiterminator	-
D0895_RS21420	4533733..4534947	-	1215	WP_072626914.1	lactonase family protein	-
D0895_RS21425	4535035..4535775	-	741	WP_187048420.1	KDGP aldolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (81-135)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12289.14 Da
• Isoelectric Point: 10.0957

>T170190 WP_004937033.1 NZ_CP060276:4531028-4531339 [Serratia ureilytica]
MHIISREPFNNATKQYPNEATALEAIYKALRRGEFRTPDELKALFPSLDRMKYKEKWWVIDVGGNHLRILFFASFETQKV
FIKHIVSHAEYDKLMQHYRRSSS

Nucleotide

• Length: 312 bp

>T170190 NZ_CP077098:c2188035-2187715 [Staphylococcus aureus]
ATGAATTGGGAAATTAAAGATTTAATGTGTGATATTGAAGTGGTAAAAGAAAAAATAAATGATGTAGCTACCAAACATGC
TTGGTTTGTTGAAGATAGATTTGTAAAAAATGAATTAGAAACAAAACGGGAACATATTAATTTTTCTGATAGCTATTTAG
AACATCGTATACAAAATGAACATACAGTTGAGTTATTACATGTGTACTTAAAAGAATTCAGTGAACTTATACAAAAATTT
CATGAAATAGAAAAAGCGTCATCAGAGAACTTTGACGAGGAATCAGATGACGCAAAGAATTCAATAAAAGTAGCAGAGTA
A

Antitoxin

Protein

• Length: 141 a.a.
• Molecular weight: 15752.07 Da
• Isoelectric Point: 5.2060

>AT170190 WP_089192287.1 NZ_CP060276:4531336-4531758 [Serratia ureilytica]
MITDAIKAADALLRAVPLLQGSHAQQDYRDALELVEYLLENDERHPLIDLLAKKIAEYEDNSAEFTAFNQRIAQLPQGVA
ALRVLMEQHQLSQSDFEHEIGKKSLVNQILNGKRSLTIPHIMALAKRFNLPPALFLPEAD

Nucleotide

• Length: 423 bp

>AT170190 NZ_CP077098:c2188247-2188038 [Staphylococcus aureus]
ATGAATAATGAACAAAAAGAAGTAATAGAACACTTGGTTTATCAACTTGAGTTAAGTGTCATGAATAATTTGGAAAGTTA
TGAACACACAGAATATGTTAATGGTATTGAAGTGGTTTCAGAGATCAGTCGTGAAAAGCACTTAGAATTGATAATGAAAT
GGTGCGCACAAGAATTAAAGAATAATTTTCAATTAGAGAAAGGAGAATAA

Structures

Toxin

• AlphaFold DB: A0A8B4GR63