Detailed information of TA system TA170189

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4467772..4468400
• Replicon: chromosome
• Accession: NZ_CP060276
• Organism: Serratia ureilytica strain CC119

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A8G2G488
• Locus tag: D0895_RS21085
• Protein ID: WP_016930174.1
• Coordinates: 4467772..4468062 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A8B4GQY7
• Locus tag: D0895_RS21090
• Protein ID: WP_015379150.1
• Coordinates: 4468077..4468400 (+)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D0895_RS21075	4464186..4466207	+	2022	WP_187048412.1	NADPH-dependent 2,4-dienoyl-CoA reductase	-
D0895_RS21080	4466402..4467541	-	1140	WP_033644533.1	23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG	-
D0895_RS21085	4467772..4468062	+	291	WP_016930174.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
D0895_RS21090	4468077..4468400	+	324	WP_015379150.1	HigA family addiction module antidote protein	Antitoxin
D0895_RS21095	4468393..4468905	+	513	WP_042785417.1	M48 family metallopeptidase	-
D0895_RS21100	4468956..4469936	+	981	WP_072626939.1	Gfo/Idh/MocA family oxidoreductase	-
D0895_RS21105	4469938..4470669	+	732	WP_033644531.1	glutamine amidotransferase	-
D0895_RS21110	4470925..4471890	+	966	WP_187048946.1	TerC family protein	-
D0895_RS21115	4472154..4473389	+	1236	WP_187048413.1	serine/threonine transporter SstT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (3-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (24-71)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11645.30 Da
• Isoelectric Point: 10.2782

>T170189 WP_016930174.1 NZ_CP060276:4467772-4468062 [Serratia ureilytica]
MIKSFRDRYLEQFYLEGRRSRLIPGALERQLARKLDMLAAAQQERDLHHPTGNYYKRLSGPFQGWSSLRVNMHWRLMFQW
RHDAAEKIYLDPHQDT

Nucleotide

• Length: 291 bp

>T170189 NZ_CP077098:c2126860-2126684 [Staphylococcus aureus]
TTGGATAGATGGTGGCTATCTGAGTATAAGGAGGTGGTGCCTATGGTGGCATTACTGAAATCTTTAGAAAGGAGACGCCT
AATGATTACAATTAGTACCATGTTGCAGTTTGGTTTATTCCTTATTGCATTGATAGGTCTAGTAATCAAGCTTATTGAAT
TAAGCAATAAAAAATAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 12141.78 Da
• Isoelectric Point: 6.2285

>AT170189 WP_015379150.1 NZ_CP060276:4468077-4468400 [Serratia ureilytica]
MLETTTREPTTVGEVLQEEYLTPLGIGQEELAKVLGVTRATVNRLCNGHRRMSVAEANLFADLFETTADFWLNLQAAHDR
WEARQADAQRVRLRPIKEILGRNITHA

Nucleotide

• Length: 324 bp

>AT170189 NZ_CP077098:2126553-2126694 [Staphylococcus aureus]
ATATATAGAAAAAGGGCAACATGCGCAAACATGTTACCCTAGTGAGCCCGTTAAAAAGACGGTGGCTATTTTAGATTAAA
GATTAAATTAATAACCATTTAACCATCGAAACCAGCCAAAGTTAGCGATGGTTATTTTTTAT

Structures

Antitoxin

• AlphaFold DB: A0A8B4GQY7