Detailed information of TA system TA170134

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 2307107..2307731
• Replicon: chromosome
• Accession: NZ_CP060244
• Organism: Entomobacter blattae strain G55GP

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: JGUZn3_RS10350
• Protein ID: WP_203413434.1
• Coordinates: 2307107..2307388 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: JGUZn3_RS10355
• Protein ID: WP_203413435.1
• Coordinates: 2307399..2307731 (+)
• Length: 111 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JGUZn3_RS10315	2304273..2304443	-	171	WP_203413427.1	hypothetical protein	-
JGUZn3_RS10320	2304452..2304766	-	315	WP_203413428.1	DUF3383 family protein	-
JGUZn3_RS10325	2304717..2305379	-	663	WP_203413429.1	hypothetical protein	-
JGUZn3_RS10330	2305363..2305791	-	429	WP_203413430.1	hypothetical protein	-
JGUZn3_RS10335	2305857..2306141	+	285	WP_203413431.1	type II toxin-antitoxin system RelE/ParE family toxin	-
JGUZn3_RS10340	2306131..2306457	+	327	WP_203413432.1	HigA family addiction module antidote protein	-
JGUZn3_RS10345	2306459..2307013	-	555	WP_203413433.1	hypothetical protein	-
JGUZn3_RS10350	2307107..2307388	+	282	WP_203413434.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JGUZn3_RS10355	2307399..2307731	+	333	WP_203413435.1	HigA family addiction module antidote protein	Antitoxin
JGUZn3_RS10360	2307764..2308198	-	435	WP_203413436.1	DUF4054 domain-containing protein	-
JGUZn3_RS10365	2308271..2308513	+	243	WP_203413437.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
JGUZn3_RS10370	2308507..2308833	+	327	WP_203413438.1	endoribonuclease MazF	-
JGUZn3_RS10375	2308852..2309073	-	222	WP_203413439.1	hypothetical protein	-
JGUZn3_RS10380	2309070..2309402	-	333	WP_203413440.1	hypothetical protein	-
JGUZn3_RS10385	2309404..2310450	-	1047	WP_203413441.1	DUF2184 domain-containing protein	-
JGUZn3_RS10390	2310465..2310983	-	519	WP_203413442.1	hypothetical protein	-
JGUZn3_RS10395	2310983..2312530	-	1548	WP_203413443.1	DUF2213 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2306459..2355532	49073

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (17-68)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10844.26 Da
• Isoelectric Point: 6.9796

>T170134 WP_203413434.1 NZ_CP060244:2307107-2307388 [Entomobacter blattae]
MTIQSFADKETQALYETDKSRKWNSLAKVAFRKLYMLDAAEQVDDLRAPPGNRLEKLSGDREGRYSIRINDQFRLCFIWT
AKGAENVEIVDYH

Nucleotide

• Length: 282 bp

>T170134 NZ_CP077089:710982-711269 [Pseudomonas tensinigenes]
ATGACCGAAAAATTCACTCGTTGGGACTCAGCCGAGTACCTCAAGACAGAAGAGGACATGGCTAACTATCTGGACGCCTG
CATGGAGGAGGCTGGAGATGACCCGGCTTTTATCGCCAAGGCACTTGGCACCATAGCGAGAGCGCGAGGCATGACTCAGG
TCGCACGTGACGCAGGGTTGTCCCGAGAGAGCCTCTACCGCGCACTATCAGGTGAAGGAAACCCCGAGTTCGGAACAATC
CTGAAAGTGGTGAAGGCGTTAGGGTTGAAGTTACACGCCAGTACTTGA

Antitoxin

Protein

• Length: 111 a.a.
• Molecular weight: 12676.71 Da
• Isoelectric Point: 10.2212

>AT170134 WP_203413435.1 NZ_CP060244:2307399-2307731 [Entomobacter blattae]
MNSLLYDVTPGVLLKEEFMQPYNLSANALAEALSVPTNRITSILSNRRSITADTAIRLSIFFKTTPEFWLNLQKIYDLRT
ERLQHEKAIRNQITPLQTILEKRSIVKQSV

Nucleotide

• Length: 333 bp

>AT170134 NZ_CP077089:710680-710985 [Pseudomonas tensinigenes]
ATGACTGAGATTCTTCGAAGTTCTACATTTTCCAACTGGCTTTTAAAGTTGGCTGATAGTCGCGCCAGGATGCGTATTCA
GGTGCGAATTGATCGTATGGCTGACGGAAATTTTGGCGATGTCAAAGCCATCGGTGAAGGGCTCAGCGAAGTCAGAATTG
ACTATGGCCCCGGATATCGAGTCTATTTCATGCAACAAGGTCGGCAAGTGGTCATCCTCCTTTGCGGCGGAGACAAGAGC
AGCCAACCCCGAGACATCAAGCAAGCCAGGCTCATCGCGGAGTCCTGGCAGGAGCAAAACCCATGA