Detailed information of TA system TA170128

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 6071145..6071740
• Replicon: chromosome
• Accession: NZ_CP060243
• Organism: Pseudomonas aeruginosa strain A-I-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A241XLJ5
• Locus tag: I5155_RS28840
• Protein ID: WP_003117425.1
• Coordinates: 6071462..6071740 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I5155_RS28835
• Protein ID: WP_003099268.1
• Coordinates: 6071145..6071450 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I5155_RS28800	6067788..6068063	-	276	WP_033896065.1	mercury resistance system periplasmic binding protein MerP	-
I5155_RS28805	6068079..6068429	-	351	WP_001294660.1	mercuric transport protein MerT	-
I5155_RS28810	6068501..6068956	+	456	WP_033896066.1	Hg(II)-responsive transcriptional regulator	-
I5155_RS28815	6069318..6070094	-	777	WP_033896079.1	hypothetical protein	-
I5155_RS28820	6070119..6070232	-	114	Protein_5702	RepA	-
I5155_RS28825	6070271..6070483	-	213	WP_025297679.1	AlpA family phage regulatory protein	-
I5155_RS28835	6071145..6071450	-	306	WP_003099268.1	HigA family addiction module antidote protein	Antitoxin
I5155_RS28840	6071462..6071740	-	279	WP_003117425.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I5155_RS28845	6072065..6074293	+	2229	WP_003113525.1	TonB-dependent receptor	-
I5155_RS28850	6074363..6075010	-	648	WP_003095021.1	carbonate dehydratase	-
I5155_RS28855	6075072..6076310	-	1239	WP_014603324.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-97)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10701.28 Da
• Isoelectric Point: 8.5576

>T170128 WP_003117425.1 NZ_CP060243:c6071740-6071462 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAARELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T170128 NZ_CP077087:c942310-942020 [Pseudomonas monsensis]
ATGACCGAAAAATTCACCCGTTTCGACGCCGCCGAATACCTCAAAACTCCCGAGGATATGGCGGCGTATCTGGACGCTTG
CTTTGATGATGATGAAGGCGATGGCGTACTCATCCGTGCAGCACTGAATGACATAGCCCGAGCTCAGGGCATGACACAGG
TGGCACGTGATGCCGGCCTGGGTCGTGAAAGTCTCTATAAAGCGCTGAGCAGCACTGGTAACCCCGAATTCGCAACTATC
ATGAAGGTCATGAAGGCTTTGGGTTTGAGATTGCACGCCGTCGCGGTTTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11213.79 Da
• Isoelectric Point: 5.0747

>AT170128 WP_003099268.1 NZ_CP060243:c6071450-6071145 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT170128 NZ_CP077087:c942616-942314 [Pseudomonas monsensis]
ATGATCGAACTTGAACGATCCCGTGTATTTTCCGAATGGCTCGACTCGTTGAAAGACATCATCGGCAAAGGCCGAATCAT
CTCCAGACTTCGAGCAGCCGAACATGGCAACTTTGGCGATTACGGTTTTGTCGGTGACACCGTTTACGAAATGCGTGTTC
ACTACGGTCCTGGTTACAGGATGTACTTCACTCGTCGAGACGAAGTGATTTATCTGCTTTTGATCGGGGGCGATAAATCG
ACACAACGGCGTGATATCAAGCGCGCCGTACAAATGGCACATAGCATCGGTAATGAGGAGTAA

Structures

Toxin

• AlphaFold DB: A0A241XLJ5