Detailed information of TA system TA170118

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 5195576..5196171
• Replicon: chromosome
• Accession: NZ_CP060242
• Organism: Pseudomonas aeruginosa strain B-I-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: I5154_RS24360
• Protein ID: WP_003113526.1
• Coordinates: 5195893..5196171 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I5154_RS24355
• Protein ID: WP_003133769.1
• Coordinates: 5195576..5195881 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I5154_RS24340	5192298..5193161	-	864	WP_023098850.1	integrase domain-containing protein	-
I5154_RS24345	5193762..5194904	-	1143	WP_023098851.1	hypothetical protein	-
I5154_RS24355	5195576..5195881	-	306	WP_003133769.1	HigA family addiction module antidote protein	Antitoxin
I5154_RS24360	5195893..5196171	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I5154_RS24365	5196500..5198728	+	2229	WP_023104079.1	TonB-dependent receptor	-
I5154_RS24370	5198799..5199446	-	648	WP_003095021.1	carbonate dehydratase	-
I5154_RS24375	5199508..5200746	-	1239	WP_003095023.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (6-82)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T170118 WP_003113526.1 NZ_CP060242:c5196171-5195893 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T170118 NZ_CP077084:c5331764-5331189 [Pseudomonas tritici]
ATGAGGTGTTCGCCTTTCACGGCCGTATACGACGCTTGTGTTCTTTACCCGGCAGCACTGCGGGATTTGCTGATGTGGCT
TGGTTTATCTGGCCTGTACCGAGCGCGGTGGACCCGTCAGATACATGAGGAGTGGAAACGTAATGTGCTTAAGAACCGAC
CAGACCTCACTCCGGATCGGGTGAATAATATCTCTGCGTTGATGGACAAGGCCATACCGGACGCGTTGGTAACTGGGTAT
GAAGATCTGTGTTCAAGCTTGGACTTACCTGATCCGGATGATCGTCATGTGCTGGCTGCAGCCATTCGGAGTAAGGCTGA
AGTCATCGTGACCTTTAATTTGAAGGACTTCCCCGCACATGTGTTGGCAGGTTATGACATAGAACCGATGCATCCGGATG
ACTTTATATCGGACCTTTGGGATTTGGATCAGGCAGCGGTATTAGCTGCGGCGCAGAAACAGAGGCGGGCGTTGGCGCGT
CCTTCGATGGATGGGGAAGGTTATCTTGAGATGCTGATGCGGCAAGGGTTGGTGCAGACCAGTAAGGTGCTTGGGCCTTA
TGAGATCATGCTGTAG

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11214.73 Da
• Isoelectric Point: 4.6081

>AT170118 WP_003133769.1 NZ_CP060242:c5195881-5195576 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGEQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT170118 NZ_CP077084:c5332231-5331761 [Pseudomonas tritici]
ATGATCACCAACCACCTCCCCAAAACGATTCTTCCACTGGAAAAAGAAGTCGAAGCCGCCGTGCAAGGCCAACGCGAATT
GGCGTCGCTGCTGTCGACCAAATTTGAAACCCAGCGCATCGACATCTTCGACAAGGAAGACAAACCCCACACGCTCGTAC
TTCCAACCTCAGCGTTGCGCCTGCTGGTCGATATCCTGGGCGAGTTGGCTATCGGCAATGCCGTCAAGGTAGTGCCGGTG
CATGCCGAATTGACCTCGCAGGAGGCTGCCGACCTGCTTAACGTTTCACGCCCGCATCTGGTCAAGATGCTGGAGGAGGG
CGCTATTCCTTTTACAAAAACGGGTCGGCATCGGCGAATTCGGTTTTCGGATTTGATGGCGTTCAAGCAGCGGCGAGATC
AGGAAAGTCAGGACGCAATGGAGGAACTGGTGCGGCAGACTCGGGAGTTGGGATTGGGGTATGACGAATGA

Structures

Toxin

• AlphaFold DB: V6ALY3