Detailed information of TA system TA170108

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5567070..5567665
• Replicon: chromosome
• Accession: NZ_CP060240
• Organism: Pseudomonas aeruginosa strain G-I-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: I5102_RS26130
• Protein ID: WP_003113526.1
• Coordinates: 5567387..5567665 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I5102_RS26125
• Protein ID: WP_003113527.1
• Coordinates: 5567070..5567375 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I5102_RS26105	5562186..5562473	-	288	WP_034061024.1	DUF5447 family protein	-
I5102_RS26110	5562649..5562921	-	273	WP_004352675.1	hypothetical protein	-
I5102_RS26115	5563568..5565811	-	2244	WP_121332485.1	NACHT domain-containing protein	-
I5102_RS26120	5565889..5566680	-	792	WP_128446579.1	hypothetical protein	-
I5102_RS26125	5567070..5567375	-	306	WP_003113527.1	HigA family addiction module antidote protein	Antitoxin
I5102_RS26130	5567387..5567665	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I5102_RS26135	5567994..5570222	+	2229	WP_023086667.1	TonB-dependent receptor	-
I5102_RS26140	5570292..5570939	-	648	WP_003095021.1	carbonate dehydratase	-
I5102_RS26145	5571001..5572239	-	1239	WP_003113524.1	dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T170108 WP_003113526.1 NZ_CP060240:c5567665-5567387 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T170108 NZ_CP077083:2452753-2453007 [Pseudomonas salmasensis]
ATGAAAATACTTTTTACCCCTGAAGGCTGGGACGACTACCTCTGGTTTCAACAGAACGACAAAGCCGGGCTCAAGCGCAT
CAACCTCTTGATCAAGGCCATACAGAGAGACCCGTTCGACGGCGTGGGAAAGCCTGAACCTCTCAAACATAATCTGAGTG
GTTTCTGGTCTCGGCGAATCACCGCGGAGCATCGTTTGGTTTATGGCATCGAAGATGATGAAGTACAAATATTGATGTGC
CGCTACCACTACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT170108 WP_003113527.1 NZ_CP060240:c5567375-5567070 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT170108 NZ_CP077083:2452502-2452756 [Pseudomonas salmasensis]
ATGGAAACTATCAACTACACCAACGCCCGAGCCCATCTGTCCGAAACGATGGACCGGGTCAACGATGATCACGTCCCACT
GTTACTGACTCGACAAAAAGGCGAGCCGGTGGTGATGATCTCGTTATCAGAGTTCAATGCTTTGGAAGAGACGGCTTACT
TGCTGCGCTCGCCTAAAAATGCCGAACGTCTCATTAACTCAGTCAACAGTTTACGAGCAGGCAAAGCCAAGCCAAGGCGG
TTGATTGAAGAATGA

Structures

Toxin

• AlphaFold DB: V6ALY3