Detailed information of TA system TA170073

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 457763..458469
• Replicon: chromosome
• Accession: NZ_CP060159
• Organism: Xylella fastidiosa subsp. fastidiosa strain GV230

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: Q87EE2
• Locus tag: XFGV230_RS01865
• Protein ID: WP_004090696.1
• Coordinates: 457763..458065 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: Q87EE1
• Locus tag: XFGV230_RS01870
• Protein ID: WP_004090697.1
• Coordinates: 458068..458469 (+)
• Length: 134 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
XFGV230_RS01830	453531..454250	-	720	Protein_342	phage late control D family protein	-
XFGV230_RS01835	454150..455121	-	972	WP_159241593.1	hypothetical protein	-
XFGV230_RS01840	455129..455686	-	558	WP_011097610.1	phage tail protein I	-
XFGV230_RS01845	455679..456572	-	894	WP_011097611.1	baseplate J/gp47 family protein	-
XFGV230_RS01850	456572..456910	-	339	WP_004090694.1	GPW/gp25 family protein	-
XFGV230_RS01855	457108..457389	+	282	WP_004090695.1	type II toxin-antitoxin system RelE/ParE family toxin	-
XFGV230_RS01860	457400..457675	+	276	WP_004085172.1	HigA family addiction module antidote protein	-
XFGV230_RS01865	457763..458065	+	303	WP_004090696.1	type II toxin-antitoxin system MqsR family toxin	Toxin
XFGV230_RS01870	458068..458469	+	402	WP_004090697.1	type II toxin-antitoxin system MqsA family antitoxin	Antitoxin
XFGV230_RS01875	458538..459125	-	588	WP_004090703.1	phage baseplate assembly protein V	-
XFGV230_RS01880	459122..459664	-	543	WP_011097613.1	hypothetical protein	-
XFGV230_RS01885	459640..460161	-	522	WP_057683415.1	hypothetical protein	-
XFGV230_RS01890	460158..460481	-	324	WP_038233035.1	hypothetical protein	-
XFGV230_RS01895	460481..460741	-	261	WP_011097616.1	hypothetical protein	-
XFGV230_RS01900	460759..462633	-	1875	WP_140161485.1	phage major capsid protein	-
XFGV230_RS01905	462617..463261	-	645	WP_038232805.1	phage portal protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	435900..468538	32638

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (2-99)

Antitoxin

• Cro/C1-type helix-turn-helix domain (74-127)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 10948.71 Da
• Isoelectric Point: 8.0251

>T170073 WP_004090696.1 NZ_CP060159:457763-458065 [Xylella fastidiosa subsp. fastidiosa]
MEKGTSHYKLPVVKALVKAGKVRATATAYQGARELGIKDLTGMCTVVLALTSADFYKSMTTYADHTVFQDVYHAKTASGD
EVYLKLTVIDDVLIVSFKEL

Nucleotide

• Length: 303 bp

>T170073 NZ_CP077078:321516-321797 [Pseudomonas azerbaijanoriens]
ATGACTTATAAGCTCGAATTTCTCCCGTCTGCACGCAAAGAATGGGACAAGCTGGGGCACACAGTGCGTGAGCAATTCAA
AAAGAAGCTGTCAGAGCGGCTTGAGGTGCCTCGAATCCAGTCTGACGCACTGCATGGCATGCCTGACTGCTACAAGATCA
AGCTGAGGTCATCGGGATATCGACTCGTCTACGAGGTGATAGATGAGCGCATTGTTGTTTCTGTCGTTGCAGTAGGAAAG
CGCGAACGCAGTGAGGTGTATGAGCGCGCCAGTAAACGTTGA

Antitoxin

Protein

• Length: 134 a.a.
• Molecular weight: 14944.02 Da
• Isoelectric Point: 7.1650

>AT170073 WP_004090697.1 NZ_CP060159:458068-458469 [Xylella fastidiosa subsp. fastidiosa]
MRCPCCGAAELIHDTRDMLYTYKSETTSIPTVTGDFCPACGEVVLDREHGDRYSELVGLFQRQVNSAYVDPGYITKVRRK
LDLDQRQAAELFGGGVNAFSRYENGKTKPPLSLVKLFKLLDRHPDLLNEVKSS

Nucleotide

• Length: 402 bp

>AT170073 NZ_CP077078:321281-321526 [Pseudomonas azerbaijanoriens]
ATGCAAAATGTTTTGGCCGATATGGCTGTCAGCGTGTCTGAACTAAAGAAAAACCCCTCTGCCGTTTTGAATGGCGCCCA
TGGAGGGCCCGTCGCGGTGCTCAATCACAATCGCGTCATGGGCTACATGGTTCCTGCCGATGTGTTCGAAGCCATGATGG
AGCGCCTTGATGATTTGGAGTTGGCGGAACTCGTGCGTTCTCGTCGTCATGAAACGCCGATCCCGGTAACGCTGGATGAC
TTATAA

Structures

Toxin

• AlphaFold DB: Q87EE2

Antitoxin

• AlphaFold DB: Q87EE1