Detailed information of TA system TA170050

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 1210574..1211091
• Replicon: chromosome
• Accession: NZ_CP060141
• Organism: Staphylococcus aureus strain 2868B2

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: T1YC79
• Locus tag: H5U44_RS05875
• Protein ID: WP_000113262.1
• Coordinates: 1210825..1211091 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: T1YB60
• Locus tag: H5U44_RS05870
• Protein ID: WP_000584499.1
• Coordinates: 1210574..1210825 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5U44_RS05840 (1206272)	1206272..1206991	+	720	WP_000479576.1	amino acid ABC transporter permease	-
H5U44_RS05845 (1206988)	1206988..1207719	+	732	WP_000615461.1	amino acid ABC transporter ATP-binding protein	-
H5U44_RS05850 (1207816)	1207816..1209066	-	1251	WP_001012223.1	FemA/FemB family glycyltransferase FmhA	-
H5U44_RS05855 (1209321)	1209321..1209683	+	363	WP_000819843.1	DUF4467 domain-containing protein	-
H5U44_RS05860 (1209702)	1209702..1210301	+	600	WP_000162813.1	DsbA family protein	-
H5U44_RS05865 (1210308)	1210308..1210409	+	102	WP_031774922.1	hypothetical protein	-
H5U44_RS05870 (1210574)	1210574..1210825	+	252	WP_000584499.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
H5U44_RS05875 (1210825)	1210825..1211091	+	267	WP_000113262.1	Txe/YoeB family addiction module toxin	Toxin
H5U44_RS05880 (1211126)	1211126..1211203	+	78	Protein_1154	hypothetical protein	-
H5U44_RS05885 (1211390)	1211390..1212940	+	1551	WP_000727763.1	zinc ABC transporter substrate-binding lipoprotein AdcA	-
H5U44_RS05890 (1213126)	1213126..1213593	+	468	WP_000153344.1	SRPBCC domain-containing protein	-
H5U44_RS05895 (1213677)	1213677..1213859	+	183	WP_000230294.1	hypothetical protein	-
H5U44_RS05900 (1214076)	1214076..1214900	+	825	WP_000572040.1	formate/nitrite transporter family protein	-
H5U44_RS05905 (1215133)	1215133..1215669	+	537	WP_031585827.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10437.95 Da
• Isoelectric Point: 9.9143

>T170050 WP_000113262.1 NZ_CP060141:1210825-1211091 [Staphylococcus aureus]
MARLNITFSPQAFEDYKYFQQNDKKMVKKINELLKSIDRNGALEGIGKPEKLKSNLTGYYSRRINHEHRLVYTVDDNHIK
IASCKYHY

Nucleotide

• Length: 267 bp

>T170050 NZ_CP077074:382022-382303 [Pseudomonas sessilinigenes]
ATGACTTATAAACTCGAGTTCCTGCCTTCGGCGCTCAAGGAGTGGAACAAGCTGGGCCATACGCTTCGCGAGCAATTCAA
GAAGAAGCTCGCGCAACGGCTGCAAATGCCCCAAGTCCCAGCAGATGCCCTGTACGGTTTACCCGACTGCTACAAGATTA
AGCTCAAGGCCTCGGGTTATCGGCTGGTCTATAAGGTTATCGAGCAGCGCGTTGTGGTGGCTGTTGTCGCGGTCGGTAAG
CGCGAGCGCGGGGCTGTGTACGAGCAAGCCAGGAAGCGTTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9433.60 Da
• Isoelectric Point: 4.3407

>AT170050 WP_000584499.1 NZ_CP060141:1210574-1210825 [Staphylococcus aureus]
MIIKNYSYARQNLKALMTKVNDDSDMVTVTSTDDKNVVIMSESDYNSMMETLYLQQNPNNAEHLAQSIADLERGKTITKD
IDV

Nucleotide

• Length: 252 bp

>AT170050 NZ_CP077074:381787-382032 [Pseudomonas sessilinigenes]
ATGCAAAGTATCCTGGCTGACAAAGCCGTCAGCGTATCTGAGTTGAAGAAGAATCCTTCAGCGGTATTGAACGGCGCTCA
AGGTGGCCCGGTGGCTGTGTTGAACCACAACCGGGTCATGGGCTACATGATTCCTGCCGAAGTTTTCGAAGCGTTGATGG
AGCGTTTGGACGATCTGGAACTGGCCGAGATCGTTCGGGCGCGTAGTCAGGAGACTCCCGTCCCGGTCAGTCTGGATGAC
TTATAA

Structures

Toxin

• PDB: 6L8G
• PDB: 6L8F
• PDB: 7CUA
• PDB: 6L8E

Antitoxin

• PDB: 6L8G
• PDB: 6L8F
• PDB: 7BWF
• PDB: 6L8E
• AlphaFold DB: T1YB60