Detailed information of TA system TA170047

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 1153612..1154135
• Replicon: chromosome
• Accession: NZ_CP060141
• Organism: Staphylococcus aureus strain 2868B2

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: T1YCD0
• Locus tag: H5U44_RS05600
• Protein ID: WP_000074043.1
• Coordinates: 1153869..1154135 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: T1YBB2
• Locus tag: H5U44_RS05595
• Protein ID: WP_000587618.1
• Coordinates: 1153612..1153869 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5U44_RS05575 (1148685)	1148685..1149878	+	1194	WP_000675407.1	MFS transporter	-
H5U44_RS05580 (1150109)	1150109..1150858	-	750	WP_000072466.1	CPBP family lipoprotein N-acylation protein LnsB	-
H5U44_RS05585 (1151053)	1151053..1152630	+	1578	WP_000143503.1	FMN-binding glutamate synthase family protein	-
H5U44_RS05590 (1152798)	1152798..1153253	-	456	WP_000732076.1	DUF1307 domain-containing protein	-
H5U44_RS05595 (1153612)	1153612..1153869	+	258	WP_000587618.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
H5U44_RS05600 (1153869)	1153869..1154135	+	267	WP_000074043.1	Txe/YoeB family addiction module toxin	Toxin
H5U44_RS05605 (1154491)	1154491..1155567	+	1077	WP_001076673.1	M42 family metallopeptidase	-
H5U44_RS05610 (1155815)	1155815..1156477	+	663	WP_000923524.1	ATP-binding cassette domain-containing protein	-
H5U44_RS05615 (1156470)	1156470..1157246	+	777	WP_000072147.1	iron export ABC transporter permease subunit FetB	-
H5U44_RS05620 (1157642)	1157642..1158829	+	1188	WP_000026194.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (4-87)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-68)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10657.45 Da
• Isoelectric Point: 10.3584

>T170047 WP_000074043.1 NZ_CP060141:1153869-1154135 [Staphylococcus aureus]
MSNYTVKIKNSAKLDLKKIKHPYLKKTFIEIVETLKNNPYKITQSFEKLEPKYLERYSRRINLQHRVVYTIDDRNKVVLI
LSAWSHYD

Nucleotide

• Length: 267 bp

>T170047 NZ_CP077073:c1848895-1848575 [Pseudomonas muyukensis]
ATGCTCTTCACCGAAACGGCCATCTTCACCAAACGCGTCCAGGAACGGCTCGACGATGAGAGCTACCGGTTGCTGCAGGT
CCGTCTGCTGATATGCCCTGATGCAGGCGATACGATCGAAGGAACGGGTGGCCTGCGCAAGATCCGGGTTGCAGCCAAAG
GGCACGGTAAACGGGGCGGGGCCAGAGTGATCTACTACCACTTGGTAACCGCATCGCAGATTGCGATGCTCTACATCTAC
CCCAACAACGAACAACCCAACCTCAATGCCGAGCAACGCAAAGCACTGAAAGCCGTCATCGAACACTGGAGCCAACCATG
A

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9762.78 Da
• Isoelectric Point: 4.1958

>AT170047 WP_000587618.1 NZ_CP060141:1153612-1153869 [Staphylococcus aureus]
MIITSPTEARKDFYQLLKNVNNNHEPIYISGNNAENNAVIIGLEDWKSIQETMYLVSTGTMDKVREREKDNSGTTNIDDI
DWDNL

Nucleotide

• Length: 258 bp

>AT170047 NZ_CP077073:c1848578-1848291 [Pseudomonas muyukensis]
ATGAATCGCTTCTTCGACGAACTGATGCAAAGCGTCGAACAGATGGACGTCATCGTCCATGGCCGGCGCCAACCAGCCCG
CCAGTTCGAGATCGATGCCCTGCAGGTCAAAAACATCCGCAAGGCGACCGGGTTGTCCCAGACGCGCTTTGCCGAAATGA
TCGATGTTCAGGTAGCGACCCTGCGCAATTGGGAACAGGGGCGCCGGGAGCCTACCGGGCCAGCCAAGGCGTTGCTGCGT
GCCATCCACAACGACCCCGAACACGTCCTGAAAGCACTCGCCCACTGA

Structures

Toxin

• AlphaFold DB: T1YCD0

Antitoxin

• AlphaFold DB: T1YBB2