Detailed information of TA system TA170020

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 3004453..3005146
• Replicon: chromosome
• Accession: NZ_CP060121
• Organism: Escherichia coli BL21

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: S1EZG2
• Locus tag: FFHJCLDM_RS14550
• Protein ID: WP_000415584.1
• Coordinates: 3004850..3005146 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: S1EBV2
• Locus tag: FFHJCLDM_RS14545
• Protein ID: WP_000650107.1
• Coordinates: 3004453..3004848 (-)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FFHJCLDM_RS14535	3000317..3002575	-	2259	WP_001281877.1	DNA topoisomerase IV subunit A	-
FFHJCLDM_RS14540	3002713..3004320	-	1608	WP_001295629.1	ABC transporter substrate-binding protein	-
FFHJCLDM_RS14545	3004453..3004848	-	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	Antitoxin
FFHJCLDM_RS14550	3004850..3005146	-	297	WP_000415584.1	type II toxin-antitoxin system toxin MqsR	Toxin
FFHJCLDM_RS14555	3005351..3005833	-	483	WP_000183505.1	transcriptional regulator	-
FFHJCLDM_RS14560	3005886..3006278	-	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
FFHJCLDM_RS14565	3006430..3007089	+	660	WP_001221493.1	two-component system response regulator QseB	-
FFHJCLDM_RS14570	3007086..3008435	+	1350	WP_000673402.1	two-component system sensor histidine kinase QseC	-
FFHJCLDM_RS14575	3008481..3008813	-	333	WP_000917684.1	DUF2645 family protein	-
FFHJCLDM_RS14580	3009132..3009713	+	582	WP_000072420.1	NADPH:quinone oxidoreductase MdaB	-
FFHJCLDM_RS14585	3009744..3010058	+	315	WP_000958598.1	antibiotic biosynthesis monooxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (5-97)

Antitoxin

• Zinc finger, MqsA-type (3-47)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11231.96 Da
• Isoelectric Point: 8.9070

>T170020 WP_000415584.1 NZ_CP060121:c3005146-3004850 [Escherichia coli BL21]
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Nucleotide

• Length: 297 bp

>T170020 NZ_CP076863:c3972012-3971629 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCGGG
AGCAGCGTACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCCGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14703.10 Da
• Isoelectric Point: 9.2136

>AT170020 WP_000650107.1 NZ_CP060121:c3004848-3004453 [Escherichia coli BL21]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Nucleotide

• Length: 396 bp

>AT170020 NZ_CP076863:c3972254-3972012 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Toxin

• PDB: 3HI2

Antitoxin

• AlphaFold DB: A0A7U9LU65