Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mqsRA (relBE)/MqsR-MqsA
Location 3004453..3005146 Replicon chromosome
Accession NZ_CP060121
Organism Escherichia coli BL21

Toxin (Protein)


Gene name mqsR Uniprot ID S1EZG2
Locus tag FFHJCLDM_RS14550 Protein ID WP_000415584.1
Coordinates 3004850..3005146 (-) Length 99 a.a.

Antitoxin (Protein)


Gene name mqsA Uniprot ID S1EBV2
Locus tag FFHJCLDM_RS14545 Protein ID WP_000650107.1
Coordinates 3004453..3004848 (-) Length 132 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FFHJCLDM_RS14535 3000317..3002575 - 2259 WP_001281877.1 DNA topoisomerase IV subunit A -
FFHJCLDM_RS14540 3002713..3004320 - 1608 WP_001295629.1 ABC transporter substrate-binding protein -
FFHJCLDM_RS14545 3004453..3004848 - 396 WP_000650107.1 type II toxin-antitoxin system antitoxin MqsA Antitoxin
FFHJCLDM_RS14550 3004850..3005146 - 297 WP_000415584.1 type II toxin-antitoxin system toxin MqsR Toxin
FFHJCLDM_RS14555 3005351..3005833 - 483 WP_000183505.1 transcriptional regulator -
FFHJCLDM_RS14560 3005886..3006278 - 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
FFHJCLDM_RS14565 3006430..3007089 + 660 WP_001221493.1 two-component system response regulator QseB -
FFHJCLDM_RS14570 3007086..3008435 + 1350 WP_000673402.1 two-component system sensor histidine kinase QseC -
FFHJCLDM_RS14575 3008481..3008813 - 333 WP_000917684.1 DUF2645 family protein -
FFHJCLDM_RS14580 3009132..3009713 + 582 WP_000072420.1 NADPH:quinone oxidoreductase MdaB -
FFHJCLDM_RS14585 3009744..3010058 + 315 WP_000958598.1 antibiotic biosynthesis monooxygenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin MqsR

(5-97)

Antitoxin

Zinc finger, MqsA-type

(3-47)


Sequences

Toxin        


Download         Length: 99 a.a.        Molecular weight: 11231.96 Da        Isoelectric Point: 8.9070

>T170020 WP_000415584.1 NZ_CP060121:c3005146-3004850 [Escherichia coli BL21]
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Download         Length: 297 bp

>T170020 NZ_CP076863:c3972012-3971629 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCGGG
AGCAGCGTACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCCGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 132 a.a.        Molecular weight: 14703.10 Da        Isoelectric Point: 9.2136

>AT170020 WP_000650107.1 NZ_CP060121:c3004848-3004453 [Escherichia coli BL21]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Download         Length: 396 bp

>AT170020 NZ_CP076863:c3972254-3972012 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 3HI2

Antitoxin

Source ID Structure
PDB 3HI2
PDB 3GA8
PDB 3O9X
PDB 2KZ8
PDB 3FMY
PDB 3GN5
AlphaFold DB A0A7U9LU65

References