Detailed information of TA system TA169999

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 256571..257265
• Replicon: chromosome
• Accession: NZ_CP060121
• Organism: Escherichia coli BL21

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: FFHJCLDM_RS01180
• Protein ID: WP_001263493.1
• Coordinates: 256867..257265 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: FFHJCLDM_RS01175
• Protein ID: WP_000554757.1
• Coordinates: 256571..256864 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FFHJCLDM_RS01155	252211..252708	+	498	WP_000006260.1	REP-associated tyrosine transposase RayT	-
FFHJCLDM_RS01160	252924..254663	-	1740	WP_001301256.1	flagellar biosynthesis protein FlhA	-
FFHJCLDM_RS01165	254608..255393	+	786	WP_000207564.1	putative lateral flagellar export/assembly protein LafU	-
FFHJCLDM_RS01170	255464..256519	+	1056	WP_001226164.1	DNA polymerase IV	-
FFHJCLDM_RS01175	256571..256864	+	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
FFHJCLDM_RS01180	256867..257265	+	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
FFHJCLDM_RS01185	257275..257727	+	453	WP_001059847.1	GNAT family N-acetyltransferase	-
FFHJCLDM_RS01190	257973..258176	+	204	Protein_230	RNA ligase RtcB family protein	-
FFHJCLDM_RS01195	258175..258696	+	522	Protein_231	peptide chain release factor H	-
FFHJCLDM_RS01200	258753..260210	-	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
FFHJCLDM_RS01205	260471..260929	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
FFHJCLDM_RS01210	261021..262265	+	1245	WP_000189541.1	esterase FrsA	-
-	261525..261605	+	81	NuclAT_13	-	-
-	261525..261605	+	81	NuclAT_13	-	-
-	261525..261605	+	81	NuclAT_13	-	-
-	261525..261605	+	81	NuclAT_13	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T169999 WP_001263493.1 NZ_CP060121:256867-257265 [Escherichia coli BL21]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T169999 NZ_CP076853:563266-563535 [Rhizobium sp. WYJ-E13]
ATGGCGTGGAGAATTGAGTTTCACCGGGCTGCGGAGCGTGAACTCGAAAAGCTCGGCCGCGACGCTGCCAGCCGCATCCT
TCGCTTTCTGAACGACCGCAATTCAAAGTTGGACGATCCAAGATCGGTCGGAGAGGCGCTGAAGGGCTCCGAGCTGGGAA
ACTTCTGGAAATACCGTGTCGGAGACTATCGCGTAATCGCTGACATCAATGATGGCGCAGTTCGCATTCTGATTGTGCGC
ATCGGCAATCGCCGCGATGTGTATCGATAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT169999 WP_000554757.1 NZ_CP060121:256571-256864 [Escherichia coli BL21]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT169999 NZ_CP076853:563058-563282 [Rhizobium sp. WYJ-E13]
ATGCTCGCTTTAAGATTGCCGCCGGAGATTGAAGCTCGCCTGGATGCGCTTGCAAAGCGCACCGGACGAAGCAAGAGTTT
CTATGCGCGGCAGGCTATTCTTGAACATCTTGACGATATGGAAGACATCTTCCTTGCCGAAAAGCGCCTTGAGGAGCTTC
GTAAGGAAGAAACCGATACTGTGCCGCTCTCGGAACTGATGGCGCGGTATGGCGTGGAGAATTGA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6