Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 250535..251076 Replicon chromosome
Accession NZ_CP060121
Organism Escherichia coli BL21

Toxin (Protein)


Gene name yafQ Uniprot ID A0A140NAP5
Locus tag FFHJCLDM_RS01140 Protein ID WP_000615981.1
Coordinates 250535..250813 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag FFHJCLDM_RS01145 Protein ID WP_000729704.1
Coordinates 250816..251076 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FFHJCLDM_RS01125 248117..248695 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
FFHJCLDM_RS01130 248901..249668 + 768 WP_000333379.1 class II glutamine amidotransferase -
FFHJCLDM_RS01135 249639..250379 - 741 WP_001225679.1 murein L,D-transpeptidase -
FFHJCLDM_RS01140 250535..250813 - 279 WP_000615981.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
FFHJCLDM_RS01145 250816..251076 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
FFHJCLDM_RS01150 251262..252035 + 774 WP_001353765.1 C40 family peptidase -
FFHJCLDM_RS01155 252211..252708 + 498 WP_000006260.1 REP-associated tyrosine transposase RayT -
FFHJCLDM_RS01160 252924..254663 - 1740 WP_001301256.1 flagellar biosynthesis protein FlhA -
FFHJCLDM_RS01165 254608..255393 + 786 WP_000207564.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10860.65 Da        Isoelectric Point: 10.0702

>T169998 WP_000615981.1 NZ_CP060121:c250813-250535 [Escherichia coli BL21]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQSSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T169998 NZ_CP076851:4221724-4222071 [Mycobacterium avium subsp. hominissuis]
ATGTCCTGGGATGTGATCCTGCTGGAGGAGGTCGAAACCTGGTACTTCTCGCTCGACGAAGACGCGATGGCAGCGGTCAC
CGGGGCCATCGACCTGCTCGAGCTGGAAGGACCAACGCTAGGCCGGCCGACGGTGGACAAGGTCAAGGGATCGAAGTTCC
ACAGCATGAAGGAGCTACGGCCAGCCGGCACCAGCATCCGGATCCTGTTCATCTTCGATCCGCGGCGTCAAGCGATCCTA
TTGCTCGGCGGGGACAAGGCAGGCAACTGGAAAAGCTGGTACGACAAGAACATTCCGATAGCTGAGCGAAGCTACGAGGA
CTGGCTCGCGACTGAGGGAGGTGAATGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT169998 WP_000729704.1 NZ_CP060121:c251076-250816 [Escherichia coli BL21]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT169998 NZ_CP076851:4222068-4222397 [Mycobacterium avium subsp. hominissuis]
ATGAGCATGGCACGCAACTGGCGTGATATCCGTGCCGACGCCATCGCGCAGGGGCGCCTGGACCCCGCCCGCGCCGATGC
CGCGCGCAACGAGATGCACGACGCGGTCCAGGCCCACCGGCTCGCCGAAATTCGCAAAGCCCAAGGCCACAGACGTCAGG
CCGACGTCGCTGTCCTGATGGGGGTTTCGCAAGCGCGAGTGTCGAAGTTGGAGAGCGGCGACCTGTCGCACACCGAGCTT
GGCACGCTCCAGTCTTACGTGGCGGCGTTGGGCGGCAGCCTCCGGGTCGTCGCCGAGTTTGACGAGAAGACCGTGGAGCT
GACAACTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MMG
PDB 4MMJ
PDB 4ML2
PDB 4ML0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References