Detailed information of TA system TA169991

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4058036..4058763
• Replicon: chromosome
• Accession: NZ_CP060117
• Organism: Shigella sonnei strain UKMCC_1015

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q3YYE6
• Locus tag: HYI41_RS20500
• Protein ID: WP_000547563.1
• Coordinates: 4058452..4058763 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HYI41_RS20495
• Protein ID: WP_000126296.1
• Coordinates: 4058036..4058455 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HYI41_RS20475 (4053087)	4053087..4053614	-	528	WP_001078777.1	electron transport protein HydN	-
HYI41_RS20480 (4053762)	4053762..4054772	-	1011	WP_001402444.1	DNA-binding transcriptional regulator AscG	-
HYI41_RS20485 (4055032)	4055032..4056489	+	1458	WP_001107890.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
HYI41_RS20490 (4056498)	4056498..4057922	+	1425	WP_000110319.1	6-phospho-beta-glucosidase AscB	-
HYI41_RS20495 (4058036)	4058036..4058455	-	420	WP_000126296.1	helix-turn-helix domain-containing protein	Antitoxin
HYI41_RS20500 (4058452)	4058452..4058763	-	312	WP_000547563.1	type II toxin-antitoxin system HigB family toxin	Toxin
HYI41_RS20505 (4058925)	4058925..4059698	-	774	WP_001026444.1	hypothetical protein	-
HYI41_RS20510 (4059723)	4059723..4060193	-	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
HYI41_RS20515 (4060186)	4060186..4060596	-	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
HYI41_RS20520 (4060593)	4060593..4061360	-	768	WP_000067403.1	formate hydrogenlyase subunit HycG	-
HYI41_RS20525 (4061360)	4061360..4061902	-	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
HYI41_RS20530 (4061912)	4061912..4063609	-	1698	WP_001288143.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12398.11 Da
• Isoelectric Point: 9.7105

>T169991 WP_000547563.1 NZ_CP060117:c4058763-4058452 [Shigella sonnei]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T169991 NZ_CP076846:c1744495-1744241 [Agrobacterium pusense]
ATGGCAAATGTGCGATTCACGGAATTCCGGCAGAACTTCGCGACCCATTTCGACCGGGTTCTGGAGACGCGCGCGCCGTT
GCTGGTTACTCGGCAGGGGAAGGAGGCGGTCGTGGTTCTCGCTGAGGGGGAATACGAGAGCATGCAGGAAACGCTGCATC
TTTTATCCAACCCGGCAAATGCCTTACGACTTCGTACTTCCATGGATCAACTTGAACGCGGCGATATCATCGAGCGGGAT
CCAACCGAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.38 Da
• Isoelectric Point: 4.3653

>AT169991 WP_000126296.1 NZ_CP060117:c4058455-4058036 [Shigella sonnei]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT169991 NZ_CP076846:c1744244-1743975 [Agrobacterium pusense]
ATGAAGCTCGTCTGGACGCTGAGTTCATGGGACGATTACGAGTTCTGGCAAAGAACCGATGCTCGTATGGTAGAGAAAAT
TAACGACCTCATACGGAATGCCAAACGGACGCCCTTTGCTGGGCTCGGAAAACCGGAGCCTCTGAAGGGCGATATGGCAG
GATATTGGTCTCGGCGGATTTCCGCCGAGCATCGGTTTGTCTATCGTGTATCCGGGTCCGGCAGCGAACAAAGGCTGGAG
ATCATCCAGTGCCGCTTCCATTACCAGTAA

Structures

Toxin

• AlphaFold DB: A0A7U9QA22