Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 1262002..1262543 Replicon chromosome
Accession NZ_CP060117
Organism Shigella sonnei strain UKMCC_1015

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag HYI41_RS06060 Protein ID WP_000615976.1
Coordinates 1262002..1262280 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag HYI41_RS06065 Protein ID WP_000729704.1
Coordinates 1262283..1262543 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HYI41_RS06045 (1259584) 1259584..1260162 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
HYI41_RS06050 (1260368) 1260368..1261135 + 768 WP_000333380.1 class II glutamine amidotransferase -
HYI41_RS06055 (1261106) 1261106..1261846 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
HYI41_RS06060 (1262002) 1262002..1262280 - 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
HYI41_RS06065 (1262283) 1262283..1262543 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
HYI41_RS06070 (1262729) 1262729..1263502 + 774 WP_127148796.1 C40 family peptidase -
HYI41_RS06080 (1264552) 1264552..1264680 + 129 Protein_1172 ISAs1 family transposase -
HYI41_RS06085 (1264751) 1264751..1265248 + 498 WP_000006258.1 REP-associated tyrosine transposase RayT -
HYI41_RS06090 (1265371) 1265371..1267083 - 1713 Protein_1174 flagellar biosynthesis protein FlhA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 1251852..1265248 13396


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T169978 WP_000615976.1 NZ_CP060117:c1262280-1262002 [Shigella sonnei]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T169978 NZ_CP076838:3201621-3201839 [Leclercia pneumoniae]
ATGTCTGAAAAACCATTAACTAAAACAGATTATTTGATGCGTCTGCGACGTTGTCAGTCAATTGATACGCTGGAGCGCGT
TATTGAAAAAAATAAATATGAGCTTTCGGATAATGAGCTGGCGGTATTTTATTCAGCAGCCGATCATCGCCTTGCAGAAC
TGACGATGAATAAGCTGTACGATAAAATCCCTACATCTGTGTGGAAATTTGTACGTTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT169978 WP_000729704.1 NZ_CP060117:c1262543-1262283 [Shigella sonnei]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT169978 NZ_CP076838:3201220-3201612 [Leclercia pneumoniae]
ATGGACGAATACTCTCCAAAAAGGCATGATATTGCGCAGTTGAAATTTCTCTGTGAATCCTTGTACCATGATTGCCTCGC
CAACCTTGATGACAGCAACCATGGCTGGGTTAATGACCCAACCTCTGCCATCAATTTGCAGTTAAACGAGCTCATTGAAC
ATATAGCGACCTTCGCTCTTAATTATAAAATTAAGTACAACGAAGATAATAAGTTGATCGAGCAAATTGACGAGTACCTG
GACGATACATTTATGCTGTTCAGTAGCTATGGCATTAACTCACAAGATTTGCAGAAATGGCGTAAATCTGGCAATCGCCT
CTTTCGCTGTTTTGTGAATGCAAGCAGAGCAAATCCTTTAGGCTCGTATGTTAAAACTATTACAAACAATTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References