Detailed information of TA system TA169951

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 7951..8567
• Replicon: plasmid p114-1
• Accession: NZ_CP060089
• Organism: Vibrio parahaemolyticus strain TJA114

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: H5404_RS24515
• Protein ID: WP_086482456.1
• Coordinates: 8280..8567 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: H5404_RS24510
• Protein ID: WP_086482455.1
• Coordinates: 7951..8268 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5404_RS24485	3478..4419	+	942	WP_086482451.1	TIGR03756 family integrating conjugative element protein	-
H5404_RS24490	4485..5732	+	1248	WP_170927767.1	integrating conjugative element protein	-
H5404_RS24495	5742..6017	+	276	WP_086482452.1	hypothetical protein	-
H5404_RS24500	6008..7588	+	1581	WP_086482453.1	conjugal transfer protein TraG N-terminal domain-containing protein	-
H5404_RS24505	7617..7862	-	246	WP_086482454.1	hypothetical protein	-
H5404_RS24510	7951..8268	-	318	WP_086482455.1	HigA family addiction module antidote protein	Antitoxin
H5404_RS24515	8280..8567	-	288	WP_086482456.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H5404_RS24520	8734..9285	-	552	WP_086482457.1	DUF5384 family protein	-
H5404_RS24525	9291..9797	-	507	WP_086482458.1	hypothetical protein	-
H5404_RS24530	9955..10548	+	594	WP_086482459.1	hypothetical protein	-
H5404_RS24535	10824..12167	+	1344	WP_086482460.1	outer membrane protein transport protein	-
H5404_RS24540	12504..12695	+	192	WP_086482461.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..120965	120965

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (12-69)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11272.91 Da
• Isoelectric Point: 7.3567

>T169951 WP_086482456.1 NZ_CP060089:c8567-8280 [Vibrio parahaemolyticus]
MAVNFKAEWLEAFYEEDKHHRLIPKTIENALFRKLEILDAAKAESDLRVPPGNRFEHLEGNLKGWCSIRVNKQYRLIFKW
VNGVAEDTYLDPHKY

Nucleotide

• Length: 288 bp

>T169951 NZ_CP076814:373908-374297 [Acinetobacter baumannii]
TTGATCAACCATTTGAATTTTAAGCTGAAATATAGTCGTTTTTCGATTATATTTCAGTTTTTTATTGGCTTAAGCTTAGC
AGTTCTATTTTATCAATTAATGCCGTTACTATGGTGGTTAGTTGTAATCAGCTTACTATTTATCAGCTTTATTTTCTTTT
TAAAGCGTCCGCAGCTTGCACAAATTGCTTATTTAGATAAAAAACTATGGTCGCTTGCTTATCATTCACAGAACACCATT
TCTCGCGTCAAAATTTTAAAAATAATTGATTATCAGATTTTCATAGTGATCTATTTTGAAGGTCATAAAAAACTAACTTC
TATCATTTGGTTTGATCAAATGTCATTAGCAGAGTGGAGAAAGCTTAAAACTTTAGAAAAACTCTATTAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11931.86 Da
• Isoelectric Point: 10.2653

>AT169951 WP_086482455.1 NZ_CP060089:c8268-7951 [Vibrio parahaemolyticus]
MRRTKRRPVSVGEMLKLEFLEPMEITSKTLAEAMGVHRNTVSNLINGGTLTAPLAIKLAAALGNTPEFWLNIQHAVDLWD
TRNRYQEEAKLVKPLFSTRDIELRT

Nucleotide

• Length: 318 bp

>AT169951 NZ_CP076814:373644-373901 [Acinetobacter baumannii]
ATGTCTGAAGAAATGACCTTGGAAGAACGTAAAGTAATCTATCGCGCACGTCGTGGTTTAAAGGAAATTGATGTCTATTT
TGACCCTTATGTGAAAAATTATTATTTAAAGGCAGACCCGGCAGAAAAAGCGTTGTTTGCTGAGTTGGTAGAACAGGAAG
ATCCTGATTTACTCGATTGGTTTATGGAAGTATCAGAACCACCACGTACCGAATTACGTGACTTTATCTATAAATTAAAA
CACTATGTTCATGGTTAA