Detailed information of TA system TA169946

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 5704024..5704619
• Replicon: chromosome
• Accession: NZ_CP060086
• Organism: Pseudomonas aeruginosa strain JNQH-PA57

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: H5409_RS26410
• Protein ID: WP_003113526.1
• Coordinates: 5704341..5704619 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: H5409_RS26405
• Protein ID: WP_003133769.1
• Coordinates: 5704024..5704329 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H5409_RS26375	5699508..5699699	+	192	WP_124105899.1	hypothetical protein	-
H5409_RS26380	5699826..5700491	-	666	WP_071534811.1	uracil-DNA glycosylase	-
H5409_RS26385	5700495..5701607	-	1113	WP_121367218.1	nucleoside triphosphate pyrophosphohydrolase family protein	-
H5409_RS26390	5701604..5702200	-	597	WP_071534809.1	hypothetical protein	-
H5409_RS26395	5702197..5702643	-	447	WP_124105900.1	hypothetical protein	-
H5409_RS26400	5702823..5703631	-	809	Protein_5218	restriction endonuclease	-
H5409_RS26405	5704024..5704329	-	306	WP_003133769.1	HigA family addiction module antidote protein	Antitoxin
H5409_RS26410	5704341..5704619	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H5409_RS26415	5704948..5707176	+	2229	WP_003141628.1	TonB-dependent receptor	-
H5409_RS26420	5707246..5707893	-	648	WP_003095021.1	carbonate dehydratase	-
H5409_RS26425	5707955..5709193	-	1239	WP_003141631.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (6-82)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T169946 WP_003113526.1 NZ_CP060086:c5704619-5704341 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T169946 NZ_CP076807:1053545-1053934 [Acinetobacter baumannii]
TTGATCAACCATTTGAACTTTAAGCTGAAATATAGTCGTTTTTCGATTATATTTCAGTTTTTTATTGGCTTAAGCTTAGC
AATTCTATTTTATCAATTAATGCCGTTACTATGGTGGTTAGTTGTAATCAGCTTACTATTTATCAGCTTTATTTTCTTTT
TAAAGCGTCCGCAGCTTGCAAAAATTGCCTATTTAGATAAAAAACTATGGTCGCTTGCTTATCACTCACAGAACACCATT
TCTCGCGTCAAAATTTTAAAAATAATTGATTATCAGATTTTCATAGTGATCTATTTTGAAGGTCATAAAACACTAACTTC
TATCATTTGGTTTGATCAAATGTCATTAGCAGAGTGGAAAAAGCTTAAAACTTTAGAAAAACTCTATTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11214.73 Da
• Isoelectric Point: 4.6081

>AT169946 WP_003133769.1 NZ_CP060086:c5704329-5704024 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGEQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT169946 NZ_CP076807:1053281-1053538 [Acinetobacter baumannii]
ATGTCTGAAGAAATGACCTTGGAAGAACGTAAAGTAATCTATCGCGCACGTCGTGGTTTAAAGGAAATTGATGTCTATTT
TGACCCTTATGTGAAAAATTATTATTTAAAGGCAGACCCGGCAGAAAAAGCGTTGTTTGCTGAGTTGGTAGAACAGGAAG
ATCCTGATTTACTCGATTGGTTTATGGAAGTATCAGAACCACCACGTACCGAATTACGTGACTTTATCTATAAATTAAAA
CACTATGTTCATGGTTAA

Structures

Toxin

• AlphaFold DB: V6ALY3