Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4089012..4089553 Replicon chromosome
Accession NZ_CP060083
Organism Escherichia coli strain G1/2

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag H4P46_RS20275 Protein ID WP_000615983.1
Coordinates 4089275..4089553 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID A0A0A0GQE3
Locus tag H4P46_RS20270 Protein ID WP_047645105.1
Coordinates 4089012..4089272 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H4P46_RS20250 4084687..4085472 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
H4P46_RS20255 4085417..4087156 + 1740 WP_001334802.1 flagellar biosynthesis protein FlhA -
H4P46_RS20260 4087380..4087877 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
H4P46_RS20265 4088053..4088802 - 750 WP_000056849.1 C40 family peptidase -
H4P46_RS20270 4089012..4089272 + 261 WP_047645105.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
H4P46_RS20275 4089275..4089553 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
H4P46_RS20280 4089709..4090449 + 741 WP_001225679.1 murein L,D-transpeptidase -
H4P46_RS20285 4090420..4091187 - 768 WP_000333380.1 class II glutamine amidotransferase -
H4P46_RS20290 4091393..4091971 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - gmhA/lpcA / rhs/PAAR / rhs/PAAR / rhs/PAAR / vgrG/tssI 4035992..4112289 76297


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T169928 WP_000615983.1 NZ_CP060083:4089275-4089553 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T169928 NZ_CP076733:133988-134926 [Photobacterium damselae]
ATGGATAGAGAGAGCCCTTATTACAAGCAGGTTTCACTGCTGATTAGAATGTTACCAGTGGTTGCAACAGAACCTTCATT
TGCACTTAAAGGTGGTACAGCTATCAACTTATTTGTGAGGGATTTTCCACGCTTGTCTGTGGATATTGATCTTGCTTATC
TTCCTCTTGAGCCCAGAAATGAAGCTCTAGCAAATGTAAAAGCAGCATTGCAACGTATAACCGATTTAATCAACAAAGAA
CCTGAGAACCGAGCTGTATTGCAGGACCATAGGTTAGATGAATTACGTATTGTTGTTACAAGTCCTAATGCAACAATTAA
AATAGAAGTCTCTCCGGTAGCAAGAGGTACGTTGCATAAACCTAATAGCTTAGCTGTCCAAGAATCAGTCGAAGAAGAGT
TTGGTTATGCAGAGATTCCTGTAGTTAGTCTGCCCGATCTTTATGGCGGAAAACTATGTGCTGCCATGGATCGCCAGCAC
CCTCGCGACCTGTTTGATGTTCGTATGCTGCTTGCAGAGGAAGGTATTTCACGAGATATCTTTGTTGGATTCATTACTTA
CGTACTAAGCCACCCAAGACCTATCAATGAGGTTATGGCTCCTAACTGGCAACCTCTTGATGATAAGTTTCAAGCTGAGT
TCGATGGCATGACCTTTGAACCTGTTGAGTTAAACGACTTAATTTCTATAAGGCCAGCGATGGTAGCTGCACTACAAGCC
CATTTCACAGAAAAAGATCGCTCATTTTTGTTGTCATTCAAACAAGGGAATCCTGATTGGTCACTGTTTGACTACCCAGA
TGCAGCAGAACTGCCAGCTATACGCTGGAAGCTACAAAACATAGCTAAGTTAGCTAAGAATCAAGAAAAACATGTAGAGC
AGCTAACTAAGTTAGAGTCTGTACTTGATGAGTGGCTTAAGCAAGCTAACTCCAATTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9386.75 Da        Isoelectric Point: 5.0608

>AT169928 WP_047645105.1 NZ_CP060083:4089012-4089272 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLHEPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT169928 NZ_CP076733:133204-133995 [Photobacterium damselae]
ATGTCATCCAAGATAAACTGGTTGGTAGCCAATGCATATCCTGGAGCCCTTGTGCTACAGCAATGGCTGACTGAGAACGG
AATTAGCTATTCCTTAGCTCAAAAATATGCGCAGAGTGGTTGGCTTAGGAAGCTATCTTCAGGCGTTTATTATCGGCCAA
GCGCAAGAGATGATGTTAAGCCGGGCTGGGTGGATGCTCTACAAGCGATGGACACACAGTTGCACCATCGTGTTCATCTG
GCTGGGTTAAGCAGCTTAACTCACCAAGGGCTGAGCCACTATTTACAGTTTAGTAAAGAACAAGTTTGGATAGGGGTGAA
GAACAAACAATCATTGCCTAAGTGGTTTCGTGAATTTCCCAATCAAAATTGGTTTTATTGTGGTAACCATAAGCTTGAAG
CTGTCTCTGACAAGGACCTAAAAGTGATTACGGTTAAAGGAAAAGAGCTAAAAGCTAGTTGCCCAGAGCTTGCTGCTTAC
GAAGTTGTTGATGCAATCGGGAAGCAAATTTCTTTTGAGCATGCAGCAGAGCTTTTTCAAGGCCTTGTAAACCTGAGTCC
TAGAAAGGTGCAATCTCTTCTTGAACGTAGTAGCTCGGTTCAAACCAATAGAGTGTTTTTGTTCTTGAGTCATTATCATG
GGCATCAGTGGACTCAGCGTATTGATGAAACAAAGATCGCATTAGGTTCAGGAAAGCGTCAGGTGGTAGAACAAGGGCGA
TTTGATGAGCGTTACCAAATCACTGTGCCAGAAATATTAAGTGTAAAAAAAGAAGAAGCAAACAATGGATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
AlphaFold DB A0A0A0GQE3

References