Detailed information of TA system TA169922

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 2532612..2533239
• Replicon: chromosome
• Accession: NZ_CP060083
• Organism: Escherichia coli strain G1/2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0Q3ID11
• Locus tag: H4P46_RS12350
• Protein ID: WP_016232083.1
• Coordinates: 2532910..2533239 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A0A0H4B4
• Locus tag: H4P46_RS12345
• Protein ID: WP_047644930.1
• Coordinates: 2532612..2532920 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H4P46_RS12305	2527724..2527924	+	201	WP_000437871.1	Cro/Cl family transcriptional regulator	-
H4P46_RS12310	2528063..2528359	+	297	WP_032257057.1	hypothetical protein	-
H4P46_RS12315	2528604..2529616	+	1013	Protein_2409	DNA-binding protein	-
H4P46_RS12320	2529623..2530363	+	741	WP_032179071.1	ATP-binding protein	-
H4P46_RS12325	2530389..2531159	+	771	WP_054518628.1	DUF1627 domain-containing protein	-
H4P46_RS12330	2531175..2531606	+	432	WP_000196185.1	DUF977 family protein	-
H4P46_RS12335	2531639..2532313	-	675	WP_032144013.1	DUF4145 domain-containing protein	-
H4P46_RS12340	2532312..2532557	+	246	WP_001624507.1	hypothetical protein	-
H4P46_RS12345	2532612..2532920	-	309	WP_047644930.1	helix-turn-helix transcriptional regulator	Antitoxin
H4P46_RS12350	2532910..2533239	-	330	WP_016232083.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H4P46_RS12355	2533559..2534230	+	672	WP_054518629.1	ORF6N domain-containing protein	-
H4P46_RS12360	2534285..2534695	+	411	WP_047645478.1	recombination protein NinB	-
H4P46_RS12365	2534692..2534883	+	192	WP_001254269.1	NinE family protein	-
H4P46_RS12370	2534907..2535197	+	291	WP_024194163.1	DUF1364 domain-containing protein	-
H4P46_RS12375	2535194..2535556	+	363	WP_021565206.1	RusA family crossover junction endodeoxyribonuclease	-
H4P46_RS12380	2535553..2535753	+	201	WP_000994506.1	protein ninH	-
H4P46_RS12385	2535988..2536602	+	615	WP_001241329.1	hypothetical protein	-
H4P46_RS12390	2536878..2537171	-	294	WP_159105021.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2491600..2655310	163710

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (15-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (44-97)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 12732.74 Da
• Isoelectric Point: 9.2605

>T169922 WP_016232083.1 NZ_CP060083:c2533239-2532910 [Escherichia coli]
MNYIIEYYSDEVEAEILSLPETLQARYIRYTEKMRIYGANLGSPHTEAFGDGLFEIRLKGSEGIGRVFYCTLKGKRIIML
HSFVKKTQKTPPAELRKAETRMKEVKHDW

Nucleotide

• Length: 330 bp

>T169922 NZ_CP076726:3267794-3267946 [Xanthomonas arboricola pv. juglandis]
ATGAAGCGACTGCTGACACTGATGGTACTGGGCCTGTTTTCGGCCGGCGTGATGACTGGCTGCAACACCATGGCCGGCGC
TGGCAAGGACATGCAGGGTGCAGGTGAGAAGGTCGAAAAGAAGGCCGACAATTGCAGCGACGGTAAGTGCTGA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11065.78 Da
• Isoelectric Point: 9.7256

>AT169922 WP_047644930.1 NZ_CP060083:c2532920-2532612 [Escherichia coli]
MTGKRTPPTMTHDDMVEKMLSNPAVKAEYDAIADEFALLDEMLAARKEAGLTQAEVAERMGTKATAITRMESNLASGTSG
PSFATLKKFARATGKKLQIRFV

Nucleotide

• Length: 309 bp

>AT169922 NZ_CP076726:3268029-3268163 [Xanthomonas arboricola pv. juglandis]
ATGAAGCGTGCAATTGTGCTGCTGGTGCTGTCGGTGTTGTCGGTCGGTATGCTGTCGGGTTGCAACACCGTCGCAGGTGC
CGGCAAGGACGTGCAGGGCGCTGGCGAGAAGGTGGAAGACGCTGCGCGCAAGTAA

Structures

Toxin

• AlphaFold DB: A0A0Q3ID11

Antitoxin

• AlphaFold DB: A0A0A0H4B4