Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1957335..1957837 Replicon chromosome
Accession NZ_CP060083
Organism Escherichia coli strain G1/2

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag H4P46_RS09330 Protein ID WP_000767829.1
Coordinates 1957583..1957837 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag H4P46_RS09325 Protein ID WP_001259255.1
Coordinates 1957335..1957586 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H4P46_RS09300 1952513..1953580 - 1068 WP_021533029.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
H4P46_RS09305 1953580..1954650 - 1071 WP_021533028.1 histidinol-phosphate transaminase -
H4P46_RS09310 1954647..1955951 - 1305 WP_047644720.1 histidinol dehydrogenase -
H4P46_RS09315 1955957..1956856 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
H4P46_RS09320 1957002..1957052 - 51 WP_001364200.1 his operon leader peptide -
H4P46_RS09325 1957335..1957586 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
H4P46_RS09330 1957583..1957837 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
H4P46_RS09335 1957920..1958744 + 825 WP_000754752.1 SDR family oxidoreductase -
H4P46_RS09340 1958790..1959719 + 930 WP_000803351.1 LysR family transcriptional regulator -
H4P46_RS09345 1959934..1959996 + 63 WP_010723108.1 membrane protein YoeI -
H4P46_RS09350 1959986..1961344 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
H4P46_RS09355 1961523..1962581 + 1059 WP_000492311.1 transport protein YeeE -
H4P46_RS09360 1962595..1962822 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T169912 WP_000767829.1 NZ_CP060083:1957583-1957837 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T169912 NZ_CP076724:c300654-300352 [Geomonas nitrogeniifigens]
ATGCCCAAAAAAGTCGTTGTATCCGACCTGCCAGAATTTGATCTTGCTATGCAGTTAAAAAGCGAAGCCGATATTGCGGC
GTATATCACCATGGTGATTGAAGAAGGTGACGCAGCAGAGTTGGCCCATGCCCTGGGAGTGGCTGCCCGAGCGCGCGGCA
TGGCCGAAATTGCGCATGCTGCCGGTTTGACTCGGGAAGCGCTATATAAAGCCTTACGTCCCGGAGCTCATCCGCGTTTC
GACACGATCAGCAGAGTTTGTTCCGCGCTCGGAGTCCGCTTGGTAGCACAGCCTCGCCATTAG

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT169912 WP_001259255.1 NZ_CP060083:1957335-1957586 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT169912 NZ_CP076724:c300954-300658 [Geomonas nitrogeniifigens]
CTGTACATCGTCAAGACCCTTCCCGAATTTGACGCTTGGCTAAATGGCATCAAAGACCGCACTACTCGGTTACGGCTGGC
TCGACGCCTTGACAAGGCCCAGCGAGGCAACCTGGGTGATGTGAAACCGGTTGGCCACGGCGTTTTCGAGATGCGTGAGT
TCTTTGGCTCCGGCTGGCGAATGTACTACATAGAACGTGGCAACATCCTTATAGTTATGCTCGGCGGTGGAGACAAGTCC
ACACAAGCAGCCGACATCGCCATTGCCTTCGAGCGGGCAGCCAGATTGGAGGATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References