Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 83732..84459 Replicon plasmid pSYM5/6
Accession NZ_CP060076
Organism Escherichia coli strain G3/10

Toxin (Protein)


Gene name higB Uniprot ID A0A663AUB1
Locus tag H4P47_RS24450 Protein ID WP_000558568.1
Coordinates 83732..84043 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID V0AHC4
Locus tag H4P47_RS24455 Protein ID WP_000990392.1
Coordinates 84040..84459 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H4P47_RS24440 79754..80335 - 582 WP_001196201.1 recombinase family protein -
H4P47_RS24445 80494..83502 + 3009 WP_000718520.1 Tn3 family transposase -
H4P47_RS24450 83732..84043 + 312 WP_000558568.1 type II toxin-antitoxin system HigB family toxin Toxin
H4P47_RS24455 84040..84459 + 420 WP_000990392.1 helix-turn-helix domain-containing protein Antitoxin
H4P47_RS24460 84496..84705 - 210 WP_020806042.1 hypothetical protein -
H4P47_RS24465 85445..85792 - 348 Protein_83 IS6-like element IS26 family transposase -
H4P47_RS24470 85825..86793 + 969 WP_000654811.1 IS5 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - - 1..100783 100783
- inside IScluster/Tn - - 74982..93662 18680


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(83-136)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12372.11 Da        Isoelectric Point: 10.0935

>T169905 WP_000558568.1 NZ_CP060076:83732-84043 [Escherichia coli]
MHVVSRAPFDTATRQFPNQAAALDDVYRTLKRENYTSPDEMKKRFASLDRMKYREKWWVIDVGGGNLRVMFFADFERGKI
FIKHITTHAEYDKLTDFYRRTKE

Download         Length: 312 bp

>T169905 NZ_CP076721:1903412-1903594 [Streptococcus parasuis]
ATGCCTATGACACAAAAAGAAATGGTTAAGCTACTTACCGCCCATGGTTGGACAAAAACCAAGGGCGGTAAGGGGTCACA
CGTGAAACTTGAAAAAGCAGGCGAGCGACCCATAACCATTCACCACGGTGAAATAAACAAATACACCGAAAGGGGTATCA
AAAAGCAAGCAGGGCTGTTATAA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15534.71 Da        Isoelectric Point: 4.4702

>AT169905 WP_000990392.1 NZ_CP060076:84040-84459 [Escherichia coli]
MMYTDAIQAANSLVSIVPLLGGNASRKDYEDALTLVEYLVEHEPDHPLVDMLVAKIAQYEDEAEEFAEFNDRIAALPSGV
ALLRVLMDQHKLTQSDFEEEIGKKSLVSRILNGTRSLTLDHMKALARRFNIPPSSFMDA

Download         Length: 420 bp

>AT169905 NZ_CP076721:1903632-1904081 [Streptococcus parasuis]
ATGCTTGTCACTTATCCAGCTTTATTTTATTTTGATGATACAGACGGAGCTAACGCACCCTACTTTGTAACATTCCCTGA
TTTTGAGCATTCAGCAACACAAGGCGAGGATATGGCGGACGCTATGGCAATGGCTAGCGATTGGTTAGGCATGAACTTAG
CTGATTATATCGAGAATGGACGAGACATTCCTACCCCGTCTTCTATAAATACTTTGTCATTAGTAGATAATAACCCTTTC
CGTGATGAGGATTTTGAGATGGTTTACGATTCTAGCAAGTCTTTTATCTCCATGGTGATGGTGGATGTTGCAAAGTATTT
GGGCAGTCAAGAGCCAGTAAAGAAAACACTCACTATCCCACGCTGGGCAGACACTCTAGGACGTGAACTAGGATTGAATT
TCTCACAGACCCTCACGGATGCAATTGCAGATAAAAAGATCCATGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A663AUB1

Antitoxin

Source ID Structure
AlphaFold DB V0AHC4

References